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学科主题: 物理化学
题名: High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase
作者: Zhang, Sufang1, 2;  Yang, Fan1;  Wang, Qian1, 2;  Hua, Yanyan1;  Zhao, Zongbao K.1, 2
通讯作者: 赵宗保
关键词: Inulinase ;  Secretory expression ;  Inulin ;  Pichia pastoris ;  Enzyme activity
刊名: PROCESS BIOCHEMISTRY
发表日期: 2012
DOI: 10.1016/j.procbio.2011.10.002
卷: 47, 期:1, 页:151-155
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering, Chemical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology ;  Engineering
英文摘要: Inulinase is an important enzyme used in the high fructose syrup and other related industries. A more cost-effective approach is required for producing highly active inulinase. In this study, the gene encoding inulinase of the yeast Kluyveromyces marxianus CBS 6556 was expressed in methylotrophic host Pichia pastoris and secretory production of recombinant inulinase (rKmINU) in the yeast under methanol induction was achieved. The purified rKmINU showed a specific activity of 2714 U/mg, which is over 12-fold higher than those of other inulinases described previously. It displayed excellent stability from 30 to 50 degrees C and pH 3.0-5.0, and the half-life of rKmINU was over 96 under these conditions. Moreover, rKmINU saccharified Jerusalem artichoke tuber juice effectively. (C) Elsevier Ltd. All rights reserved.
关键词[WOS]: YEAST PICHIA-PASTORIS ;  ASPERGILLUS-NIGER ;  EXTRACELLULAR INULINASE ;  GENE ;  PURIFICATION ;  CLONING ;  PROTEINS ;  CBS-6556
语种: 英语
WOS记录号: WOS:000300133000022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118274
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.CAS, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China

Recommended Citation:
Zhang, Sufang,Yang, Fan,Wang, Qian,et al. High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase[J]. PROCESS BIOCHEMISTRY,2012,47(1):151-155.
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