DICP OpenIR
Subject Area物理化学
High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase
Zhang, Sufang1,2; Yang, Fan1; Wang, Qian1,2; Hua, Yanyan1; Zhao, Zongbao K.1,2; Zhao ZB(赵宗保)
KeywordInulinase Secretory Expression Inulin Pichia Pastoris Enzyme Activity
Source PublicationPROCESS BIOCHEMISTRY
2012
DOI10.1016/j.procbio.2011.10.002
Volume47Issue:1Pages:151-155
Indexed BySCI
SubtypeArticle
Department18
Funding Project1816
Contribution Rank1,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
WOS KeywordYEAST PICHIA-PASTORIS ; ASPERGILLUS-NIGER ; EXTRACELLULAR INULINASE ; GENE ; PURIFICATION ; CLONING ; PROTEINS ; CBS-6556
AbstractInulinase is an important enzyme used in the high fructose syrup and other related industries. A more cost-effective approach is required for producing highly active inulinase. In this study, the gene encoding inulinase of the yeast Kluyveromyces marxianus CBS 6556 was expressed in methylotrophic host Pichia pastoris and secretory production of recombinant inulinase (rKmINU) in the yeast under methanol induction was achieved. The purified rKmINU showed a specific activity of 2714 U/mg, which is over 12-fold higher than those of other inulinases described previously. It displayed excellent stability from 30 to 50 degrees C and pH 3.0-5.0, and the half-life of rKmINU was over 96 under these conditions. Moreover, rKmINU saccharified Jerusalem artichoke tuber juice effectively. (C) Elsevier Ltd. All rights reserved.
Language英语
WOS IDWOS:000300133000022
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118274
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhao ZB(赵宗保)
Affiliation1.CAS, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Sufang,Yang, Fan,Wang, Qian,et al. High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase[J]. PROCESS BIOCHEMISTRY,2012,47(1):151-155.
APA Zhang, Sufang,Yang, Fan,Wang, Qian,Hua, Yanyan,Zhao, Zongbao K.,&赵宗保.(2012).High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase.PROCESS BIOCHEMISTRY,47(1),151-155.
MLA Zhang, Sufang,et al."High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase".PROCESS BIOCHEMISTRY 47.1(2012):151-155.
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