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学科主题: 物理化学
题名: A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides
作者: Zhao, Liang1;  Qin, Hongqiang1;  Hu, Zhengyan1;  Zhang, Yi1;  Wu, Ren'an1;  Zou, Hanfa1
通讯作者: 吴仁安 ;  邹汉法
刊名: CHEMICAL SCIENCE
发表日期: 2012
DOI: 10.1039/c2sc20363d
卷: 3, 期:9, 页:2828-2838
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 1,1
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Immobilized metal affinity chromatography (IMAC) is a powerful method in phosphopeptide enrichment. However, the achievement of highly specific enrichment and sensitive detection of phosphopeptide by IMAC is still a big challenge because of the lack of high specificity and large binding capacity of conventional IMAC materials. Here, we report a novel IMAC nanoparticle to dramatically improve the enrichment specificity for the phosphopeptide by introducing a titanium phosphate moiety on a poly(ethylene glycol) methacrylate (PEG) brush decorated Fe3O4@SiO2 core-shell nanoparticle (denoted as Fe3O4@SiO2@PEG-Ti4+ IMAC nanoparticle). The thicker grafting layer of the PEG brushes has a higher chelating capacity of titanium ions. Due to the combination of the superior nonfouling property and the enhanced binding capacity of the grafted PEG brushes, the Fe3O4@SiO2@PEG-Ti4+ IMAC nanoparticle demonstrated a high phosphopeptide recovery (over 70%) and low limit of detection (0.5 fmol), along with an exceptional great specificity to capture phosphopeptides from a tryptic digest of the mixture of a nonphosphorylated protein BSA and a phosphorylated protein alpha-casein with molar ratios of BSA/alpha-casein up to 2000 : 1. In the analysis of a real complex biological sample, the tryptic digests of Arabidopsis, 2447 unique phosphopeptides have been identified, showing a superior performance of the Fe3O4@SiO2@PEG-Ti4+ IMAC nanoparticle than that of Fe3O4@SiO2-Ti4+ (1186) and commercial TiO2 microspheres (961). We believe that the PEG decoration for IMAC materials will be a convenient approach to significantly improve the specificity and the binding capacity of phosphopeptide enrichment.
关键词[WOS]: METAL AFFINITY-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ANALYSIS ;  MALDI-TOF-MS ;  MULTI-PHOSPHORYLATED PEPTIDES ;  PHOSPHOPROTEOME ANALYSIS ;  BIOMEDICAL APPLICATIONS ;  RADICAL POLYMERIZATION ;  SELECTIVE ENRICHMENT ;  SILICA MICROSPHERES ;  RAPID ENRICHMENT
语种: 英语
WOS记录号: WOS:000306931300023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118291
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Zhao, Liang,Qin, Hongqiang,Hu, Zhengyan,et al. A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides[J]. CHEMICAL SCIENCE,2012,3(9):2828-2838.
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