DICP OpenIR
Subject Area物理化学
A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides
Zhao, Liang; Qin, Hongqiang; Hu, Zhengyan; Zhang, Yi; Wu, Ren'an; Zou, Hanfa; Wu RA(吴仁安); Zou HF(邹汉法)
Source PublicationCHEMICAL SCIENCE
2012
ISSN2041-6520
DOI10.1039/c2sc20363d
Volume3Issue:9Pages:2828-2838
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank1,1
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordMETAL AFFINITY-CHROMATOGRAPHY ; MASS-SPECTROMETRY ANALYSIS ; MALDI-TOF-MS ; MULTI-PHOSPHORYLATED PEPTIDES ; PHOSPHOPROTEOME ANALYSIS ; BIOMEDICAL APPLICATIONS ; RADICAL POLYMERIZATION ; SELECTIVE ENRICHMENT ; SILICA MICROSPHERES ; RAPID ENRICHMENT
AbstractImmobilized metal affinity chromatography (IMAC) is a powerful method in phosphopeptide enrichment. However, the achievement of highly specific enrichment and sensitive detection of phosphopeptide by IMAC is still a big challenge because of the lack of high specificity and large binding capacity of conventional IMAC materials. Here, we report a novel IMAC nanoparticle to dramatically improve the enrichment specificity for the phosphopeptide by introducing a titanium phosphate moiety on a poly(ethylene glycol) methacrylate (PEG) brush decorated Fe3O4@SiO2 core-shell nanoparticle (denoted as Fe3O4@SiO2@PEG-Ti4+ IMAC nanoparticle). The thicker grafting layer of the PEG brushes has a higher chelating capacity of titanium ions. Due to the combination of the superior nonfouling property and the enhanced binding capacity of the grafted PEG brushes, the Fe3O4@SiO2@PEG-Ti4+ IMAC nanoparticle demonstrated a high phosphopeptide recovery (over 70%) and low limit of detection (0.5 fmol), along with an exceptional great specificity to capture phosphopeptides from a tryptic digest of the mixture of a nonphosphorylated protein BSA and a phosphorylated protein alpha-casein with molar ratios of BSA/alpha-casein up to 2000 : 1. In the analysis of a real complex biological sample, the tryptic digests of Arabidopsis, 2447 unique phosphopeptides have been identified, showing a superior performance of the Fe3O4@SiO2@PEG-Ti4+ IMAC nanoparticle than that of Fe3O4@SiO2-Ti4+ (1186) and commercial TiO2 microspheres (961). We believe that the PEG decoration for IMAC materials will be a convenient approach to significantly improve the specificity and the binding capacity of phosphopeptide enrichment.
Language英语
WOS IDWOS:000306931300023
Citation statistics
Cited Times:74[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118291
Collection中国科学院大连化学物理研究所
Corresponding AuthorWu RA(吴仁安); Zou HF(邹汉法)
AffiliationChinese Acad Sci, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Liang,Qin, Hongqiang,Hu, Zhengyan,et al. A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides[J]. CHEMICAL SCIENCE,2012,3(9):2828-2838.
APA Zhao, Liang.,Qin, Hongqiang.,Hu, Zhengyan.,Zhang, Yi.,Wu, Ren'an.,...&邹汉法.(2012).A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides.CHEMICAL SCIENCE,3(9),2828-2838.
MLA Zhao, Liang,et al."A poly(ethylene glycol)-brush decorated magnetic polymer for highly specific enrichment of phosphopeptides".CHEMICAL SCIENCE 3.9(2012):2828-2838.
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