中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein
作者: Zhang, Wei1;  He, Xi-Wen1;  Chen, Yang1;  Li, Wen-You1;  Zhang, Yu-Kui1, 2
通讯作者: 李文友
关键词: Molecular imprinting ;  Quantum dots ;  Denatured bovine serum albumin ;  Artificial receptors ;  Selective protein recognition
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2012-01-15
DOI: 10.1016/j.bios.2011.09.042
卷: 31, 期:1, 页:84-89
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 2,5
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: A new type of molecularly imprinted polymer (MIP)-based fluorescent artificial receptor was developed by anchoring MIP on the surface of denatured bovine serum albumin (dBSA) modified CdTe quantum dots (QDs) using the surface molecular imprinting process. The approach combined the merits of molecular imprinting technology and the fluorescent property of the CdTe QDs. The dBSA was used not only to modify the surface defects of the CdTe QDs, but also as assistant monomer to create effective recognition sites. Three different proteins, namely lysozyme (Lyz), cytochrome c (Cyt) and methylated bovine serum albumin (mBSA), were tested as the template molecules and then the receptors were synthesized by sol-gel reaction (imprinting process). The results of fluorescence and binding experiments demonstrated the recognition performance of the receptors toward the corresponding template. Under optimum conditions, the linear range for Lyz was from 1.4 x 10(-8) to 8.5 x 10(-6) M, and the detection limit was 6.8 nM. Moreover, the new artificial receptors were applied to separate and detect Lyz in real samples. This fluorescent artificial receptor may serve as a starting point in the design of highly effective synthetic fluorescent receptor for recognition of target protein. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: NANOPARTICLES ;  NANOCRYSTALS ;  SENSOR ;  WATER ;  PHOTOLUMINESCENCE ;  BIOMOLECULES ;  LYSOZYME ;  SYSTEM
语种: 英语
WOS记录号: WOS:000300468400013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118297
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2012bmlMT6dC8S.PDF(621KB)----开放获取--View Download

作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Zhang, Wei,He, Xi-Wen,Chen, Yang,et al. Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein[J]. BIOSENSORS & BIOELECTRONICS,2012,31(1):84-89.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhang, Wei]'s Articles
 [He, Xi-Wen]'s Articles
 [Chen, Yang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhang, Wei]‘s Articles
 [He, Xi-Wen]‘s Articles
 [Chen, Yang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2012bmlMT6dC8S.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace