DICP OpenIR
Subject Area物理化学
Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein
Zhang, Wei1; He, Xi-Wen1; Chen, Yang1; Li, Wen-You1; Zhang, Yu-Kui1,2; Li WY(李文友)
KeywordMolecular Imprinting Quantum Dots Denatured Bovine Serum Albumin Artificial Receptors Selective Protein Recognition
Source PublicationBIOSENSORS & BIOELECTRONICS
2012-01-15
DOI10.1016/j.bios.2011.09.042
Volume31Issue:1Pages:84-89
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank2,5
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS KeywordNANOPARTICLES ; NANOCRYSTALS ; SENSOR ; WATER ; PHOTOLUMINESCENCE ; BIOMOLECULES ; LYSOZYME ; SYSTEM
AbstractA new type of molecularly imprinted polymer (MIP)-based fluorescent artificial receptor was developed by anchoring MIP on the surface of denatured bovine serum albumin (dBSA) modified CdTe quantum dots (QDs) using the surface molecular imprinting process. The approach combined the merits of molecular imprinting technology and the fluorescent property of the CdTe QDs. The dBSA was used not only to modify the surface defects of the CdTe QDs, but also as assistant monomer to create effective recognition sites. Three different proteins, namely lysozyme (Lyz), cytochrome c (Cyt) and methylated bovine serum albumin (mBSA), were tested as the template molecules and then the receptors were synthesized by sol-gel reaction (imprinting process). The results of fluorescence and binding experiments demonstrated the recognition performance of the receptors toward the corresponding template. Under optimum conditions, the linear range for Lyz was from 1.4 x 10(-8) to 8.5 x 10(-6) M, and the detection limit was 6.8 nM. Moreover, the new artificial receptors were applied to separate and detect Lyz in real samples. This fluorescent artificial receptor may serve as a starting point in the design of highly effective synthetic fluorescent receptor for recognition of target protein. (C) 2011 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000300468400013
Citation statistics
Cited Times:82[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118297
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi WY(李文友)
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Wei,He, Xi-Wen,Chen, Yang,et al. Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein[J]. BIOSENSORS & BIOELECTRONICS,2012,31(1):84-89.
APA Zhang, Wei,He, Xi-Wen,Chen, Yang,Li, Wen-You,Zhang, Yu-Kui,&李文友.(2012).Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein.BIOSENSORS & BIOELECTRONICS,31(1),84-89.
MLA Zhang, Wei,et al."Molecularly imprinted polymer anchored on the surface of denatured bovine serum albumin modified CdTe quantum dots as fluorescent artificial receptor for recognition of target protein".BIOSENSORS & BIOELECTRONICS 31.1(2012):84-89.
Files in This Item:
File Name/Size DocType Version Access License
2012bmlMT6dC8S.PDF(621KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Wei]'s Articles
[He, Xi-Wen]'s Articles
[Chen, Yang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Wei]'s Articles
[He, Xi-Wen]'s Articles
[Chen, Yang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Wei]'s Articles
[He, Xi-Wen]'s Articles
[Chen, Yang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.