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学科主题: 物理化学
题名: A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses
作者: Wang, Xu2, 3, 4, 5;  Bian, Yangyang1;  Cheng, Kai1;  Zou, Hanfa1;  Sun, Samuel Sai-Ming4, 5;  He, Jun-Xian4, 5
通讯作者: 邹汉法 ;  SamuelSaiMingSun ;  JunXianHe
关键词: proteomics ;  phosphoproteomics ;  nitrogen (N) ;  nitrate ;  N signaling ;  N use efficiency ;  Arabidopsis thaliana
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2012-04-01
DOI: 10.1021/pr2010764
卷: 11, 期:4, 页:2301-2315
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 4,2
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Nitrogen (N) is an important nutrient and signal for plant growth and development. However, to date, our knowledge of how plants sense and transduce the N signals is very limited. To better understand the molecular mechanisms of plant N responses, we took two-dimensional gel-based proteomic and phosphoproteomic approaches to profile the proteins with abundance and phosphorylation state changes during nitrate deprivation and recovery in the model plant Arabidopsis thaliana. After 7-day-old seedlings were N-deprived for up to 48 h followed by 24 h recovery, a total of 170 and 38 proteins were identified with significant changes in abundance and phosphorylation state, respectively. Bioinformatic analyses implicate these proteins in diverse cellular processes including N and protein metabolisms, photosynthesis, cytoskeleton, redox homeostasis, and signal transduction. Functional studies of the selected nitrate-responsive proteins indicate that the proteasome regulatory subunit RPT5a and the cytoskeleton protein Tubulin alpha-6 (TUA6) play important roles in plant nit-rate responses by regulating plant N use efficiency (NUE) and low nitrate-induced anthocyanin biosynthesis, respectively. In conclusion, our study provides novel insights into plant responses to nitrate at the proteome level, which are expected to be highly useful for dissecting the N response pathways in higher plants and for improving plant NUE.
关键词[WOS]: GENE-EXPRESSION ;  AMINO-ACIDS ;  ROOT ;  THALIANA ;  ASSIMILATION ;  METABOLISM ;  BIOSYNTHESIS ;  LIMITATION ;  DATABASE ;  GROWTH
语种: 英语
WOS记录号: WOS:000302388100023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118307
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
3.Tsinghua Univ, Div Life Sci, Grad Sch Shenzhen, Shenzhen 518055, Guangdong, Peoples R China
4.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong, Peoples R China
5.Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China

Recommended Citation:
Wang, Xu,Bian, Yangyang,Cheng, Kai,et al. A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(4):2301-2315.
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