DICP OpenIR
Subject Area物理化学
A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses
Wang, Xu2,3,4,5; Bian, Yangyang1; Cheng, Kai1; Zou, Hanfa1; Sun, Samuel Sai-Ming4,5; He, Jun-Xian4,5; Zou HF(邹汉法); SamuelSaiMingSun; JunXianHe
KeywordProteomics Phosphoproteomics Nitrogen (n) Nitrate n Signaling n Use Efficiency Arabidopsis Thaliana
Source PublicationJOURNAL OF PROTEOME RESEARCH
2012-04-01
ISSN1535-3893
DOI10.1021/pr2010764
Volume11Issue:4Pages:2301-2315
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank4,2
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordGENE-EXPRESSION ; AMINO-ACIDS ; ROOT ; THALIANA ; ASSIMILATION ; METABOLISM ; BIOSYNTHESIS ; LIMITATION ; DATABASE ; GROWTH
AbstractNitrogen (N) is an important nutrient and signal for plant growth and development. However, to date, our knowledge of how plants sense and transduce the N signals is very limited. To better understand the molecular mechanisms of plant N responses, we took two-dimensional gel-based proteomic and phosphoproteomic approaches to profile the proteins with abundance and phosphorylation state changes during nitrate deprivation and recovery in the model plant Arabidopsis thaliana. After 7-day-old seedlings were N-deprived for up to 48 h followed by 24 h recovery, a total of 170 and 38 proteins were identified with significant changes in abundance and phosphorylation state, respectively. Bioinformatic analyses implicate these proteins in diverse cellular processes including N and protein metabolisms, photosynthesis, cytoskeleton, redox homeostasis, and signal transduction. Functional studies of the selected nitrate-responsive proteins indicate that the proteasome regulatory subunit RPT5a and the cytoskeleton protein Tubulin alpha-6 (TUA6) play important roles in plant nit-rate responses by regulating plant N use efficiency (NUE) and low nitrate-induced anthocyanin biosynthesis, respectively. In conclusion, our study provides novel insights into plant responses to nitrate at the proteome level, which are expected to be highly useful for dissecting the N response pathways in higher plants and for improving plant NUE.
Language英语
WOS IDWOS:000302388100023
Citation statistics
Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118307
Collection中国科学院大连化学物理研究所
Corresponding AuthorZou HF(邹汉法); SamuelSaiMingSun; JunXianHe
Affiliation1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China
3.Tsinghua Univ, Div Life Sci, Grad Sch Shenzhen, Shenzhen 518055, Guangdong, Peoples R China
4.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Shatin, Hong Kong, Peoples R China
5.Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xu,Bian, Yangyang,Cheng, Kai,et al. A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(4):2301-2315.
APA Wang, Xu.,Bian, Yangyang.,Cheng, Kai.,Zou, Hanfa.,Sun, Samuel Sai-Ming.,...&JunXianHe.(2012).A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses.JOURNAL OF PROTEOME RESEARCH,11(4),2301-2315.
MLA Wang, Xu,et al."A Comprehensive Differential Proteomic Study of Nitrate Deprivation in Arabidopsis Reveals Complex Regulatory Networks of Plant Nitrogen Responses".JOURNAL OF PROTEOME RESEARCH 11.4(2012):2301-2315.
Files in This Item:
File Name/Size DocType Version Access License
2012azVfCwm3fr.PDF(822KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Xu]'s Articles
[Bian, Yangyang]'s Articles
[Cheng, Kai]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Xu]'s Articles
[Bian, Yangyang]'s Articles
[Cheng, Kai]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Xu]'s Articles
[Bian, Yangyang]'s Articles
[Cheng, Kai]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.