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学科主题物理化学
Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids
Chang, Yuwei; Zhao, Chunxia; Wu, Zeming; Zhou, Jia; Zhao, Sumin; Lu, Xin; Xu, Guowang; Zhao CX(赵春霞)
关键词Flavonoids Hplc-chip Ms Metabolic Profiling Soybean
刊名ELECTROPHORESIS
2012-08-01
DOI10.1002/elps.201100581
33期:15页:2399-2406
收录类别SCI
文章类型Article
部门归属18
项目归属1808
产权排名1, 1
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]INTEGRATED MICROFLUIDIC DEVICE ; ANTIOXIDANT ACTIVITY ; ISOFLAVONE CONTENT ; PHENOLIC-COMPOUNDS ; ION-SOURCE ; QUERCETIN ; IDENTIFICATION ; KAEMPFEROL ; SEEDS ; NARINGENIN
英文摘要In this work a chip-based nano HPLC coupled MS (HPLC-chip/MS) method with a simple sample preparation procedure was developed for the flavonoid profiling of soybean. The analytical properties of the method including the linearity (R2, 0.9920.995), reproducibility (RSD, 1.507.66%), intraday precision (RSD, 1.415.14%) and interday precision (RSD, 2.7616.90%) were satisfactory. Compared with the conventional HPLC/MS method, a fast extraction and analysis procedure was applied and more flavonoids were detected in a single run. Additionally, 13 flavonoids in soybean seed were identified for the first time. The method was then applied to the profiling of six varieties of soybean sowed at the same place. A clear discrimination was observed among different cultivars, three isoflavones, accounting for nearly 80% of total flavonoid contents, were found increased in the spring soybeans compared with the summer cultivars.
语种英语
WOS记录号WOS:000307399400018
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/118316
专题中国科学院大连化学物理研究所
通讯作者Zhao CX(赵春霞)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Chang, Yuwei,Zhao, Chunxia,Wu, Zeming,et al. Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids[J]. ELECTROPHORESIS,2012,33(15):2399-2406.
APA Chang, Yuwei.,Zhao, Chunxia.,Wu, Zeming.,Zhou, Jia.,Zhao, Sumin.,...&赵春霞.(2012).Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids.ELECTROPHORESIS,33(15),2399-2406.
MLA Chang, Yuwei,et al."Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids".ELECTROPHORESIS 33.15(2012):2399-2406.
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