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学科主题: 物理化学
题名: Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids
作者: Chang, Yuwei1;  Zhao, Chunxia1;  Wu, Zeming1;  Zhou, Jia1;  Zhao, Sumin1;  Lu, Xin1;  Xu, Guowang1
通讯作者: 赵春霞
关键词: Flavonoids ;  HPLC-chip ;  MS ;  Metabolic profiling ;  Soybean
刊名: ELECTROPHORESIS
发表日期: 2012-08-01
DOI: 10.1002/elps.201100581
卷: 33, 期:15, 页:2399-2406
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1, 1
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this work a chip-based nano HPLC coupled MS (HPLC-chip/MS) method with a simple sample preparation procedure was developed for the flavonoid profiling of soybean. The analytical properties of the method including the linearity (R2, 0.9920.995), reproducibility (RSD, 1.507.66%), intraday precision (RSD, 1.415.14%) and interday precision (RSD, 2.7616.90%) were satisfactory. Compared with the conventional HPLC/MS method, a fast extraction and analysis procedure was applied and more flavonoids were detected in a single run. Additionally, 13 flavonoids in soybean seed were identified for the first time. The method was then applied to the profiling of six varieties of soybean sowed at the same place. A clear discrimination was observed among different cultivars, three isoflavones, accounting for nearly 80% of total flavonoid contents, were found increased in the spring soybeans compared with the summer cultivars.
关键词[WOS]: INTEGRATED MICROFLUIDIC DEVICE ;  ANTIOXIDANT ACTIVITY ;  ISOFLAVONE CONTENT ;  PHENOLIC-COMPOUNDS ;  ION-SOURCE ;  QUERCETIN ;  IDENTIFICATION ;  KAEMPFEROL ;  SEEDS ;  NARINGENIN
语种: 英语
WOS记录号: WOS:000307399400018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118316
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Chang, Yuwei,Zhao, Chunxia,Wu, Zeming,et al. Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids[J]. ELECTROPHORESIS,2012,33(15):2399-2406.
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