DICP OpenIR
Subject Area物理化学
Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids
Chang, Yuwei; Zhao, Chunxia; Wu, Zeming; Zhou, Jia; Zhao, Sumin; Lu, Xin; Xu, Guowang; Zhao CX(赵春霞)
KeywordFlavonoids Hplc-chip Ms Metabolic Profiling Soybean
Source PublicationELECTROPHORESIS
2012-08-01
DOI10.1002/elps.201100581
Volume33Issue:15Pages:2399-2406
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1, 1
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordINTEGRATED MICROFLUIDIC DEVICE ; ANTIOXIDANT ACTIVITY ; ISOFLAVONE CONTENT ; PHENOLIC-COMPOUNDS ; ION-SOURCE ; QUERCETIN ; IDENTIFICATION ; KAEMPFEROL ; SEEDS ; NARINGENIN
AbstractIn this work a chip-based nano HPLC coupled MS (HPLC-chip/MS) method with a simple sample preparation procedure was developed for the flavonoid profiling of soybean. The analytical properties of the method including the linearity (R2, 0.9920.995), reproducibility (RSD, 1.507.66%), intraday precision (RSD, 1.415.14%) and interday precision (RSD, 2.7616.90%) were satisfactory. Compared with the conventional HPLC/MS method, a fast extraction and analysis procedure was applied and more flavonoids were detected in a single run. Additionally, 13 flavonoids in soybean seed were identified for the first time. The method was then applied to the profiling of six varieties of soybean sowed at the same place. A clear discrimination was observed among different cultivars, three isoflavones, accounting for nearly 80% of total flavonoid contents, were found increased in the spring soybeans compared with the summer cultivars.
Language英语
WOS IDWOS:000307399400018
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118316
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhao CX(赵春霞)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Chang, Yuwei,Zhao, Chunxia,Wu, Zeming,et al. Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids[J]. ELECTROPHORESIS,2012,33(15):2399-2406.
APA Chang, Yuwei.,Zhao, Chunxia.,Wu, Zeming.,Zhou, Jia.,Zhao, Sumin.,...&赵春霞.(2012).Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids.ELECTROPHORESIS,33(15),2399-2406.
MLA Chang, Yuwei,et al."Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids".ELECTROPHORESIS 33.15(2012):2399-2406.
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