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学科主题物理化学
Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides
Jin, Guojie1,4; Yang, Fan1,3; Hu, Cuimin1; Shen, Hongwei1,2; Zhao, Zongbao K.1,2; Zhao ZB(赵宗保)
关键词Biodiesel Enzyme-assisted Extraction Microbial Lipids Oleaginous Yeast
刊名BIORESOURCE TECHNOLOGY
2012-05-01
DOI10.1016/j.biortech.2012.01.152
111页:378-382
收录类别SCI
文章类型Article
部门归属18
项目归属1816
产权排名1,1
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Technology
类目[WOS]Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
研究领域[WOS]Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
关键词[WOS]WALL LYTIC ENZYME ; CELL WALL ; OPTIMIZATION ; PURIFICATION ; OIL ; MICROORGANISMS ; FERMENTATION ; HYDROLYSIS
英文摘要Lipids produced by oleaginous microorganisms are a potential feedstock for biodiesel production and chemical synthesis. Yet, the costs of microbial lipids remain high, partially because the lipid recovery process is tedious and costly. In the present study, enzyme-assisted extraction of lipids from the culture of the yeast Rhodosporidium toruloides was carried out. With a heat pre-treatment with microwave, enzymatic treatment with the recombinant beta-1,3-glucomannanase, plMAN5C, and extraction with ethyl acetate, 96.6% of the total lipids were extracted from R. toruloides cells at room temperature and atmospheric pressure directly from the culture without dewatering. Therefore, this process could significantly reduce energy consumption and costs for lipids extraction from the yeast. (C) 2012 Elsevier Ltd. All rights reserved.
语种英语
WOS记录号WOS:000302979100052
引用统计
被引频次:48[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/118318
专题中国科学院大连化学物理研究所
通讯作者Zhao ZB(赵宗保)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
3.Dalian Polytech Univ, Dalian 116034, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
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Jin, Guojie,Yang, Fan,Hu, Cuimin,et al. Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides[J]. BIORESOURCE TECHNOLOGY,2012,111:378-382.
APA Jin, Guojie,Yang, Fan,Hu, Cuimin,Shen, Hongwei,Zhao, Zongbao K.,&赵宗保.(2012).Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides.BIORESOURCE TECHNOLOGY,111,378-382.
MLA Jin, Guojie,et al."Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides".BIORESOURCE TECHNOLOGY 111(2012):378-382.
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