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学科主题: 物理化学
题名: Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides
作者: Jin, Guojie1, 4;  Yang, Fan1, 3;  Hu, Cuimin1;  Shen, Hongwei1, 2;  Zhao, Zongbao K.1, 2
通讯作者: 赵宗保
关键词: Biodiesel ;  Enzyme-assisted extraction ;  Microbial lipids ;  Oleaginous yeast
刊名: BIORESOURCE TECHNOLOGY
发表日期: 2012-05-01
DOI: 10.1016/j.biortech.2012.01.152
卷: 111, 页:378-382
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 1,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Agricultural Engineering ;  Biotechnology & Applied Microbiology ;  Energy & Fuels
研究领域[WOS]: Agriculture ;  Biotechnology & Applied Microbiology ;  Energy & Fuels
英文摘要: Lipids produced by oleaginous microorganisms are a potential feedstock for biodiesel production and chemical synthesis. Yet, the costs of microbial lipids remain high, partially because the lipid recovery process is tedious and costly. In the present study, enzyme-assisted extraction of lipids from the culture of the yeast Rhodosporidium toruloides was carried out. With a heat pre-treatment with microwave, enzymatic treatment with the recombinant beta-1,3-glucomannanase, plMAN5C, and extraction with ethyl acetate, 96.6% of the total lipids were extracted from R. toruloides cells at room temperature and atmospheric pressure directly from the culture without dewatering. Therefore, this process could significantly reduce energy consumption and costs for lipids extraction from the yeast. (C) 2012 Elsevier Ltd. All rights reserved.
关键词[WOS]: WALL LYTIC ENZYME ;  CELL WALL ;  OPTIMIZATION ;  PURIFICATION ;  OIL ;  MICROORGANISMS ;  FERMENTATION ;  HYDROLYSIS
语种: 英语
WOS记录号: WOS:000302979100052
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118318
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
3.Dalian Polytech Univ, Dalian 116034, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Jin, Guojie,Yang, Fan,Hu, Cuimin,et al. Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides[J]. BIORESOURCE TECHNOLOGY,2012,111:378-382.
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