DICP OpenIR
学科主题物理化学
Prefractionation and separation by C8 stationary phase: Effective strategies for integral membrane proteins analysis
Zhao, Qun1,2; Sun, Liangliang1,2; Liang, Yu1,2; Wu, Qi1,2; Yuan, Huiming1; Liang, Zhen1; Zhang, Lihua1; Zhan, Yukui1; Zhang LH(张丽华)
关键词Reverse Phase Stationary Phase Hydrophobic Peptides Integral Membrane Protein Prefractionation Mu Rplc-esi-ms/ms
刊名TALANTA
2012-01-15
DOI10.1016/j.talanta.2011.11.035
88页:567-572
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1,1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; SHOTGUN PROTEOMIC ANALYSIS ; LIQUID-CHROMATOGRAPHY ; ELEVATED-TEMPERATURE ; LC-MS/MS ; DIGESTION ; ENRICHMENT ; BRAIN ; IDENTIFICATION ; SOLUBILIZATION
英文摘要Analysis of integral membrane proteins (IMPS) presents great challenges due to their hydrophobic nature. Recently, much attention has been paid to improve the solubilization of IMPs. However, besides that, the separation of hydrophobic peptides with high recovery is also a dominating factor, but with rare report. Here, the prefractionation of the digests by reverse phase trap column during desalting was presented to efficiently decrease the complexity of samples, with the identified hydrophobic peptides and IMPs increased by more than 43%. Furthermore, the effect of C18 and C8 stationary phases on the separation of membrane protein digests was studied. A total of 301 proteins (536 peptides) with C18 stationary phase and 398 proteins (703 peptides) with C8 stationary phase were identified by mu RPLC-ESI-MS/MS using an LCQ instrument in duplicate runs, with false discovery rate (FDR) less than 5% at peptide level. In addition, with C8 stationary phase, the number of identified hydrophobic peptides and IMPs was obviously improved by 29% and 20%, respectively, compared with that identified by C18 stationary phase, indicating that the polarity of stationary phase has evident effect on the analysis of membrane protein digests. All these results show that the prefractionation by reverse phase trap column during desalting and the separation by C8 stationary phases could facilitate the efficient identification of IMPs. (C) 2011 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000301159400079
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/118330
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Qun,Sun, Liangliang,Liang, Yu,et al. Prefractionation and separation by C8 stationary phase: Effective strategies for integral membrane proteins analysis[J]. TALANTA,2012,88:567-572.
APA Zhao, Qun.,Sun, Liangliang.,Liang, Yu.,Wu, Qi.,Yuan, Huiming.,...&张丽华.(2012).Prefractionation and separation by C8 stationary phase: Effective strategies for integral membrane proteins analysis.TALANTA,88,567-572.
MLA Zhao, Qun,et al."Prefractionation and separation by C8 stationary phase: Effective strategies for integral membrane proteins analysis".TALANTA 88(2012):567-572.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
20129Zq3u50uWP.PDF(687KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, Qun]的文章
[Sun, Liangliang]的文章
[Liang, Yu]的文章
百度学术
百度学术中相似的文章
[Zhao, Qun]的文章
[Sun, Liangliang]的文章
[Liang, Yu]的文章
必应学术
必应学术中相似的文章
[Zhao, Qun]的文章
[Sun, Liangliang]的文章
[Liang, Yu]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。