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学科主题物理化学
Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion
Yuan, Huiming1; Zhou, Yuan1,2; Xia, Simin1,2; Zhang, Lihua1; Zhang, Xiaodan1; Wu, Qi1,2; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
刊名ANALYTICAL CHEMISTRY
2012-06-05
DOI10.1021/ac3006796
84期:11页:5124-5132
收录类别SCI
文章类型Article
部门归属18
项目归属1810
产权排名1,1
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; TRYPSIN REACTOR ; IDENTIFICATION ; MICROREACTOR ; PROTEOLYSIS ; POLYMER ; ESI
英文摘要An online integrated platform for proteome profiling was established, with the combination of protein separation by microreversed phase liquid chromatography (mu RPLC), online acetonitrile (ACN) removal, and pH adjustment by a hollow fiber membrane interface (HFMI), online digestion by an immobilized enzymatic microreactor (IMER), as well as peptide separation and proteins identification by mu RPLC or nano-RPLC-electrospray ionization tandem mass spectrometry (mu RPLC-ESI-MS/MS). To evaluate the performance of such a platform, a three-protein mixture with mass ranging from 5 to 500 ng was analyzed automatically. Compared to the offline counterpart, although similar protein sequence coverages were obtained by the integrated platform, the signal intensity of total ion chromatogram was improved by almost 4 times. In addition, such an integrated platform was further applied for the analysis of extracted proteins from rat brain. Compared to the results obtained by offline counterpart and traditional MudPIT approach under similar conditions, by the integrated platform, the identified protein group number was comparable, but the analysis time was shortened to less than half of that taken by the traditional approaches. All these results demonstrated that our developed integrated platform might offer a promising tool for high-throughput and large-scale profiling of proteomes.
语种英语
WOS记录号WOS:000304783100070
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/118362
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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GB/T 7714
Yuan, Huiming,Zhou, Yuan,Xia, Simin,et al. Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion[J]. ANALYTICAL CHEMISTRY,2012,84(11):5124-5132.
APA Yuan, Huiming.,Zhou, Yuan.,Xia, Simin.,Zhang, Lihua.,Zhang, Xiaodan.,...&张丽华.(2012).Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion.ANALYTICAL CHEMISTRY,84(11),5124-5132.
MLA Yuan, Huiming,et al."Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion".ANALYTICAL CHEMISTRY 84.11(2012):5124-5132.
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