DICP OpenIR
Subject Area物理化学
Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion
Yuan, Huiming1; Zhou, Yuan1,2; Xia, Simin1,2; Zhang, Lihua1; Zhang, Xiaodan1; Wu, Qi1,2; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
Source PublicationANALYTICAL CHEMISTRY
2012-06-05
DOI10.1021/ac3006796
Volume84Issue:11Pages:5124-5132
Indexed BySCI
SubtypeArticle
Department18
Funding Project1810
Contribution Rank1,1
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; TRYPSIN REACTOR ; IDENTIFICATION ; MICROREACTOR ; PROTEOLYSIS ; POLYMER ; ESI
AbstractAn online integrated platform for proteome profiling was established, with the combination of protein separation by microreversed phase liquid chromatography (mu RPLC), online acetonitrile (ACN) removal, and pH adjustment by a hollow fiber membrane interface (HFMI), online digestion by an immobilized enzymatic microreactor (IMER), as well as peptide separation and proteins identification by mu RPLC or nano-RPLC-electrospray ionization tandem mass spectrometry (mu RPLC-ESI-MS/MS). To evaluate the performance of such a platform, a three-protein mixture with mass ranging from 5 to 500 ng was analyzed automatically. Compared to the offline counterpart, although similar protein sequence coverages were obtained by the integrated platform, the signal intensity of total ion chromatogram was improved by almost 4 times. In addition, such an integrated platform was further applied for the analysis of extracted proteins from rat brain. Compared to the results obtained by offline counterpart and traditional MudPIT approach under similar conditions, by the integrated platform, the identified protein group number was comparable, but the analysis time was shortened to less than half of that taken by the traditional approaches. All these results demonstrated that our developed integrated platform might offer a promising tool for high-throughput and large-scale profiling of proteomes.
Language英语
WOS IDWOS:000304783100070
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118362
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yuan, Huiming,Zhou, Yuan,Xia, Simin,et al. Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion[J]. ANALYTICAL CHEMISTRY,2012,84(11):5124-5132.
APA Yuan, Huiming.,Zhou, Yuan.,Xia, Simin.,Zhang, Lihua.,Zhang, Xiaodan.,...&张丽华.(2012).Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion.ANALYTICAL CHEMISTRY,84(11),5124-5132.
MLA Yuan, Huiming,et al."Integrated Platform for Proteome Profiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion".ANALYTICAL CHEMISTRY 84.11(2012):5124-5132.
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