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学科主题: 物理化学
题名: Alteration of Leaf Metabolism in Bt-Transgenic Rice (Oryza sativa L.) and Its Wild Type under Insecticide Stress
作者: Zhou, Jia1;  Zhang, Lei2, 3;  Chang, Yuwei1;  Lu, Xin1;  Zhu, Zhen2, 3;  Xu, Guowang1
通讯作者: 朱桢 ;  许国旺
关键词: metabolomics ;  insecticide ;  rice ;  GC-MS ;  Bt-transgenic
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2012-08-01
DOI: 10.1021/pr300495x
卷: 11, 期:8, 页:4351-4360
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 1, 1
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Insecticide is always used to control the damage from pests, while the potential influence on plants is rarely known. Time-course metabolic changes of wild and Bacillus thuringiensis (Bt) transgenic rice (Oryza sativa L.) plants after insecticide treatment were investigated by using gas chromatography-mass spectrometry (GC-MS). A combined statistical strategy of 2-way ANOVA and multivariate analyses (principal component analysis and hierarchal cluster analysis) was performed to find the stress-associated effects. The results reveal that a wide range of metabolites were dynamically varied in both varieties as a response to insecticide, in multiple metabolic pathways, such as biosynthesis and metabolism of amino acids, carbohydrates, fatty acids, TCA cycle, and the shikimate/phenylpropanoid pathway, and most of the changes were correlated with the exposure time and dependent on the variety. A set of stress defenses were activated, including phytohormone signaling pathway, antioxidant defense system, shilcimate-mediated secondary metabolism, and so on. In particular, insecticide led to much stronger regulations of signaling molecules (salicylate and the precursor of jasmonate) and antioxidants (alpha-tocopherol and dehydroascorbate/ascorbate) in Bt-transgenic variety at the early stage. Our results demonstrated that the Bt-transgenic rice had a more acute and drastic response to insecticide stress than its non-transgenic counterpart in antioxidant system and signaling regulation.
关键词[WOS]: CHROMATOGRAPHY-MASS SPECTROMETRY ;  SIMULTANEOUS COMPONENT ANALYSIS ;  METABOLOMICS DATA ;  JASMONIC ACID ;  TOXICITY ;  PLANTS ;  RESPONSES ;  BIOSYNTHESIS ;  ACCUMULATION ;  CHLORPYRIFOS
语种: 英语
WOS记录号: WOS:000307037600039
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118363
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

Recommended Citation:
Zhou, Jia,Zhang, Lei,Chang, Yuwei,et al. Alteration of Leaf Metabolism in Bt-Transgenic Rice (Oryza sativa L.) and Its Wild Type under Insecticide Stress[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(8):4351-4360.
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