DICP OpenIR
Subject Area物理化学
Alteration of Leaf Metabolism in Bt-Transgenic Rice (Oryza sativa L.) and Its Wild Type under Insecticide Stress
Zhou, Jia1; Zhang, Lei2,3; Chang, Yuwei1; Lu, Xin1; Zhu, Zhen2,3; Xu, Guowang1; Zhu Z(朱桢); Xu GW(许国旺)
KeywordMetabolomics Insecticide Rice Gc-ms Bt-transgenic
Source PublicationJOURNAL OF PROTEOME RESEARCH
2012-08-01
DOI10.1021/pr300495x
Volume11Issue:8Pages:4351-4360
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank1, 1
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordCHROMATOGRAPHY-MASS SPECTROMETRY ; SIMULTANEOUS COMPONENT ANALYSIS ; METABOLOMICS DATA ; JASMONIC ACID ; TOXICITY ; PLANTS ; RESPONSES ; BIOSYNTHESIS ; ACCUMULATION ; CHLORPYRIFOS
AbstractInsecticide is always used to control the damage from pests, while the potential influence on plants is rarely known. Time-course metabolic changes of wild and Bacillus thuringiensis (Bt) transgenic rice (Oryza sativa L.) plants after insecticide treatment were investigated by using gas chromatography-mass spectrometry (GC-MS). A combined statistical strategy of 2-way ANOVA and multivariate analyses (principal component analysis and hierarchal cluster analysis) was performed to find the stress-associated effects. The results reveal that a wide range of metabolites were dynamically varied in both varieties as a response to insecticide, in multiple metabolic pathways, such as biosynthesis and metabolism of amino acids, carbohydrates, fatty acids, TCA cycle, and the shikimate/phenylpropanoid pathway, and most of the changes were correlated with the exposure time and dependent on the variety. A set of stress defenses were activated, including phytohormone signaling pathway, antioxidant defense system, shilcimate-mediated secondary metabolism, and so on. In particular, insecticide led to much stronger regulations of signaling molecules (salicylate and the precursor of jasmonate) and antioxidants (alpha-tocopherol and dehydroascorbate/ascorbate) in Bt-transgenic variety at the early stage. Our results demonstrated that the Bt-transgenic rice had a more acute and drastic response to insecticide stress than its non-transgenic counterpart in antioxidant system and signaling regulation.
Language英语
WOS IDWOS:000307037600039
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118363
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhu Z(朱桢); Xu GW(许国旺)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Jia,Zhang, Lei,Chang, Yuwei,et al. Alteration of Leaf Metabolism in Bt-Transgenic Rice (Oryza sativa L.) and Its Wild Type under Insecticide Stress[J]. JOURNAL OF PROTEOME RESEARCH,2012,11(8):4351-4360.
APA Zhou, Jia.,Zhang, Lei.,Chang, Yuwei.,Lu, Xin.,Zhu, Zhen.,...&许国旺.(2012).Alteration of Leaf Metabolism in Bt-Transgenic Rice (Oryza sativa L.) and Its Wild Type under Insecticide Stress.JOURNAL OF PROTEOME RESEARCH,11(8),4351-4360.
MLA Zhou, Jia,et al."Alteration of Leaf Metabolism in Bt-Transgenic Rice (Oryza sativa L.) and Its Wild Type under Insecticide Stress".JOURNAL OF PROTEOME RESEARCH 11.8(2012):4351-4360.
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