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学科主题: 物理化学
题名: Improved Catalytic Performance of Lipase Accommodated in the Mesoporous Silicas with Polymer-Modified Microenvironment
作者: Liu, Jia1, 2;  Bai, Shiyang1, 2;  Jin, Qianru1, 2;  Zhong, Hua1;  Li, Can1;  Yang, Qihua1
通讯作者: 杨启华 ;  李灿
刊名: LANGMUIR
发表日期: 2012-06-26
DOI: 10.1021/la301330s
卷: 28, 期:25, 页:9788-9796
收录类别: SCI
文章类型: Article
部门归属: 5
项目归属: 506;503
产权排名: 1,1
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Multidisciplinary ;  Chemistry, Physical ;  Materials Science, Multidisciplinary
研究领域[WOS]: Chemistry ;  Materials Science
英文摘要: The highly ordered mesoporous silicas with elaborately controlled microenvironment were synthesized via covalent incorporation of long-chain polymers (M-w = 2000 g mol(-1)) bearing specific hydrophilic/hydrophobic balance. The microenvironment (hydrophilicity/hydrophobicity) of the mesoporous silicas was quantitatively determined by gas adsorption experiments and investigated by lysozyme (LYZ) adsorption. The relative activity of lipase from Pseudomonas cepacia (PCL) encapsulated in the mesoporous silica with moderate hydrophobic microenvironment (hereafter denoted as MHM) reaches up to 281% compared with the free PCL, notably higher than that of PCL accommodated in the mesoporous silicas with hydrophilic or strong hydrophobic microenvironment (20.7-26.2% relative to the free PCL). Moreover, PCL entrapped in the nanochannels with MHM affords the highest initial rate in the kinetic resolution of (R,S)-1-phenylethanol relative to other immobilized PCL. The above results suggest that the MHM could render the active center of PCL entirely exposed to the substrates without interrupting its native conformation in the "interfacial activation". In addition, the nano channels with MHM could markedly improve the thermal stability of PCL (preserving nearly 60% of the initial activity after the incubation at 70 degrees C for 2 h) and facilitate the recycling of the immobilized PCL in both aqueous and organic media. Our work demonstrates that the subtle modulation of the microenvironment of mesoporous silicas for enzyme immobilization designates a very promising strategy to fabricate the highly active and stable heterogeneous biocatalysts for industrial application.
关键词[WOS]: RHIZOMUCOR-MIEHEI LIPASE ;  SOL-GEL MATERIALS ;  LYSOZYME ADSORPTION ;  INTERFACIAL ADSORPTION ;  HYDROPHOBIC SUPPORTS ;  IMMOBILIZATION ;  ENZYMES ;  HYPERACTIVATION ;  BIOCATALYSIS
语种: 英语
WOS记录号: WOS:000305661400073
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118384
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Liu, Jia,Bai, Shiyang,Jin, Qianru,et al. Improved Catalytic Performance of Lipase Accommodated in the Mesoporous Silicas with Polymer-Modified Microenvironment[J]. LANGMUIR,2012,28(25):9788-9796.
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