DICP OpenIR
Subject Area物理化学
SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis
Chu, Lingluo3; Zhu, Tongge1,3; Liu, Xing3,5; Yu, Ruoying3; Bacanamwo, Methode5; Dou, Zhen1,3; Chu, Youjun1,3; Zou, Hanfa4; Gibbons, Gary H.5; Wang, Dongmei3; Ding, Xia1,5; Yao, Xuebiao3; XiaDing; XuebiaoYao
KeywordMitosis Suv39h1 Methylation Marc Syntelin
Source PublicationJOURNAL OF MOLECULAR CELL BIOLOGY
2012-10-01
DOI10.1093/jmcb/mjs023
Volume4Issue:5Pages:331-340
Indexed BySCI
SubtypeArticle
Department18
Funding Project1809
Contribution Rank3,8
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectCell Biology
WOS Research AreaCell Biology
WOS KeywordAURORA-B KINASE ; CENP-E ; MITOTIC CHECKPOINT ; SPINDLE MICROTUBULES ; CELL-DIVISION ; PROTEIN E ; PHOSPHORYLATION ; KINETOCHORE ; ATTACHMENT ; CANCER
AbstractHistone methylation performs multiple functions such as DNA replication, transcription regulation, heterochromatin formation, and chromatin condensation. How this methylation gradient is orchestrated in the centromere during chromosome segregation is not known. Here we examine the temporal dynamics of protein methylation in the centromere by SUV39H1 methyltransferase, a key mitotic regulator, using fluorescence resonance energy transfer-based sensors in living HeLa cells and immunofluorescence of native SUV39H1 substrates. A quantitative analysis of methylation dynamics, using centromere-targeted sensors, reveals a temporal change during chromosome segregation. These dynamics result in an accurate chromosome congression to and alignment at the equator as an inhibition of methylation dynamics using SUV39H1 inhibitor perturbs chromosome congression in living HeLa cells. Surprisingly, this inhibition of methylation results in a brief increase in Aurora B kinase activity and an enrichment of microtubule depolymerase MCAK in the centromere with a concomitant kinetochoremicrotubule destabilization and a reduced tension across the sister kinetochores with ultimate chromosome misalignments. We reason that SUV39H1 generates a gradient of methylation marks at the kinetochore that provides spatiotemporal information essential for accurate chromosome segregation in mitosis.
Language英语
WOS IDWOS:000309707000007
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118427
Collection中国科学院大连化学物理研究所
Corresponding AuthorXiaDing; XuebiaoYao
Affiliation1.Beijing Univ Chinese Med, Beijing 100027, Peoples R China
2.Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Sch Life Sci, Anhua 230026, Peoples R China
3.Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
4.Dalian Inst Phys Chem, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
5.Morehouse Sch Med, Dept Physiol, Atlanta, GA 30310 USA
Recommended Citation
GB/T 7714
Chu, Lingluo,Zhu, Tongge,Liu, Xing,et al. SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2012,4(5):331-340.
APA Chu, Lingluo.,Zhu, Tongge.,Liu, Xing.,Yu, Ruoying.,Bacanamwo, Methode.,...&XuebiaoYao.(2012).SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis.JOURNAL OF MOLECULAR CELL BIOLOGY,4(5),331-340.
MLA Chu, Lingluo,et al."SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis".JOURNAL OF MOLECULAR CELL BIOLOGY 4.5(2012):331-340.
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