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学科主题: 物理化学
题名: SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis
作者: Chu, Lingluo3;  Zhu, Tongge1, 3;  Liu, Xing3, 5;  Yu, Ruoying3;  Bacanamwo, Methode5;  Dou, Zhen1, 3;  Chu, Youjun1, 3;  Zou, Hanfa4;  Gibbons, Gary H.5;  Wang, Dongmei3;  Ding, Xia1, 5;  Yao, Xuebiao3
通讯作者: XiaDing ;  XuebiaoYao
关键词: mitosis ;  SUV39H1 ;  methylation ;  MARC ;  syntelin
刊名: JOURNAL OF MOLECULAR CELL BIOLOGY
发表日期: 2012-10-01
DOI: 10.1093/jmcb/mjs023
卷: 4, 期:5, 页:331-340
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 3,8
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
英文摘要: Histone methylation performs multiple functions such as DNA replication, transcription regulation, heterochromatin formation, and chromatin condensation. How this methylation gradient is orchestrated in the centromere during chromosome segregation is not known. Here we examine the temporal dynamics of protein methylation in the centromere by SUV39H1 methyltransferase, a key mitotic regulator, using fluorescence resonance energy transfer-based sensors in living HeLa cells and immunofluorescence of native SUV39H1 substrates. A quantitative analysis of methylation dynamics, using centromere-targeted sensors, reveals a temporal change during chromosome segregation. These dynamics result in an accurate chromosome congression to and alignment at the equator as an inhibition of methylation dynamics using SUV39H1 inhibitor perturbs chromosome congression in living HeLa cells. Surprisingly, this inhibition of methylation results in a brief increase in Aurora B kinase activity and an enrichment of microtubule depolymerase MCAK in the centromere with a concomitant kinetochoremicrotubule destabilization and a reduced tension across the sister kinetochores with ultimate chromosome misalignments. We reason that SUV39H1 generates a gradient of methylation marks at the kinetochore that provides spatiotemporal information essential for accurate chromosome segregation in mitosis.
关键词[WOS]: AURORA-B KINASE ;  CENP-E ;  MITOTIC CHECKPOINT ;  SPINDLE MICROTUBULES ;  CELL-DIVISION ;  PROTEIN E ;  PHOSPHORYLATION ;  KINETOCHORE ;  ATTACHMENT ;  CANCER
语种: 英语
WOS记录号: WOS:000309707000007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118427
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Univ Chinese Med, Beijing 100027, Peoples R China
2.Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Sch Life Sci, Anhua 230026, Peoples R China
3.Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
4.Dalian Inst Phys Chem, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
5.Morehouse Sch Med, Dept Physiol, Atlanta, GA 30310 USA

Recommended Citation:
Chu, Lingluo,Zhu, Tongge,Liu, Xing,et al. SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2012,4(5):331-340.
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