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学科主题: 物理化学
题名: Metabonomics study of the acute graft rejection in rat renal transplantation using reversed-phase liquid chromatography and hydrophilic interaction chromatography coupled with mass spectrometry
作者: Chen, Jihong2;  Wen, Hao3;  Liu, Jian2;  Yu, Che2;  Zhao, Xinjie1;  Shi, Xianzhe1;  Xu, Guowang1
通讯作者: 赵欣捷
刊名: MOLECULAR BIOSYSTEMS
发表日期: 2012
DOI: 10.1039/c2mb05454j
卷: 8, 期:3, 页:871-878
收录类别: SCI
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 3,5
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Acute graft rejection is one of the most common and serious post complications in renal transplantation, noninvasive diagnosis of acute graft rejection is essential for reducing risk of surgery and timely treatment. In this study, a non-targeted metabonomics approach based on ultra performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (MS) is used to investigate the effect of acute graft rejection in rat renal transplantation on metabolism. To collect more metabolite information both hydrophilic interaction chromatography and reversed-phase liquid chromatography were used. Using the partial least squares-discriminant analysis, we found that the change of metabonome in a sham-operated group and a non-graft rejection group had a similar trend, while that of the acute graft rejection group was clearly different. Several discriminating metabolites of the acute graft rejection were identified, including creatinine, phosphatidyl-cholines, lyso-phosphatidylcholines, carnitine C16:0, free fatty acids and indoxyl sulfate etc. These discriminating metabolites suggested that acute graft rejection in renal transplantation can lead to the accumulation of creatinine in the body, and also the abnormal metabolism of phospholipids. These findings are useful to understand the mechanisms of the rejection, it also means that a UPLC-MS metabonomic approach is a suitable tool to investigate the metabolic abnormality in the acute graft rejection in renal transplantation.
关键词[WOS]: LOW-PROTEIN DIET ;  ISCHEMIA/REPERFUSION INJURY ;  ALLOGRAFT REJECTION ;  KIDNEY-TRANSPLANTS ;  INDOXYL SULFATE ;  FREE-RADICALS ;  UREMIC TOXIN ;  METABOLOMICS ;  URINE ;  PROGRESSION
语种: 英语
WOS记录号: WOS:000300048500022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118431
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, CAS Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Xinjiang Med Univ, Dept Nephrol, Affiliated Hosp 1, Urumqi 830054, Peoples R China
3.Xinjiang Med Univ, Xinjiang Organ Transplantat Res Inst, Affiliated Hosp 1, Urumqi 830054, Peoples R China

Recommended Citation:
Chen, Jihong,Wen, Hao,Liu, Jian,et al. Metabonomics study of the acute graft rejection in rat renal transplantation using reversed-phase liquid chromatography and hydrophilic interaction chromatography coupled with mass spectrometry[J]. MOLECULAR BIOSYSTEMS,2012,8(3):871-878.
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