DICP OpenIR
Subject Area物理化学
Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts
Wu, Rui2; Wu, Zeming1; Wang, Xianhui2; Yang, Pengcheng2; Yu, Dan2; Zhao, Chunxia1; Xu, Guowang1; Kang, Le2; Xu GW(许国旺); LeKang
KeywordAggregation Metabolic Profiling Phase Change
Source PublicationPROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2012-02-28
DOI10.1073/pnas.1119155109
Volume109Issue:9Pages:3259-3263
Indexed BySCI
SubtypeArticle
Department18
Funding Project1808
Contribution Rank2,2
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordACETYL-L-CARNITINE ; RAT-BRAIN ; BEHAVIOR ; METHYLATION ; INSECTS ; TOOL
AbstractPhenotypic plasticity occurs prevalently and plays a vital role in adaptive evolution. However, the underlying molecular mechanisms responsible for the expression of alternate phenotypes remain unknown. Here, a density-dependent phase polyphenism of Locusta migratoria was used as the study model to identify key signaling molecules regulating the expression of phenotypic plasticity. Metabolomic analysis, using high-performance liquid chromatography and gas chromatography-mass spectrometry, showed that solitarious and gregarious locusts have distinct metabolic profiles in hemolymph. A total of 319 metabolites, many of which are involved in lipid metabolism, differed significantly in concentration between the phases. In addition, the time course of changes in the metabolic profiles of locust hemolymph that accompany phase transition was analyzed. Carnitine and its acyl derivatives, which are involved in the lipid beta-oxidation process, were identified as key differential metabolites that display robust correlation with the time courses of phase transition. RNAi silencing of two key enzymes from the carnitine system, carnitine acetyltransferase and palmitoyltransferase, resulted in a behavioral transition from the gregarious to solitarious phase and the corresponding changes of metabolic profiles. In contrast, the injection of exogenous acetylcarnitine promoted the acquisition of gregarious behavior in solitarious locusts. These results suggest that carnitines mediate locust phase transition possibly through modulating lipid metabolism and influencing the nervous system of the locusts.
Language英语
WOS IDWOS:000300828200021
Citation statistics
Cited Times:54[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118435
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺); LeKang
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Wu, Rui,Wu, Zeming,Wang, Xianhui,et al. Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2012,109(9):3259-3263.
APA Wu, Rui.,Wu, Zeming.,Wang, Xianhui.,Yang, Pengcheng.,Yu, Dan.,...&LeKang.(2012).Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,109(9),3259-3263.
MLA Wu, Rui,et al."Metabolomic analysis reveals that carnitines are key regulatory metabolites in phase transition of the locusts".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.9(2012):3259-3263.
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