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学科主题: 物理化学
题名: Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism
作者: Zhang, Dapeng1;  Shen, Hujun2;  Li, Guohui2;  Zhao, Bailin1;  Yu, Anchi4;  Zhao, Qiang3;  Wang, Hailin1
通讯作者: WangHailin
刊名: ANALYTICAL CHEMISTRY
发表日期: 2012-09-18
DOI: 10.1021/ac302320x
卷: 84, 期:18, 页:8088-8094
收录类别: SCI
文章类型: Article
部门归属: 11
项目归属: 1106
产权排名: 2,2
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Fluorescence anisotropy (FA) is a homogeneous, ratiometric, and real-time analytical technology. By selective labeling of a guanine (G)-quadruplex motif with tetramethylrhodamine (TMR), here, it is established that a large reduction in FA response can be specifically associated with the unfolding -> folding transition of G-quadruplex structures. On the basis of fluorescence intensity, polarization and lifetime analysis, and molecular docking simulation, the mechanism was found to be that the labeled fluorophore (TMR) can intramolecularly interact with adjacent G bases in an unfolded G-quadruplex motif, which allows for the photoinduced electron transfer (PET) occurring between the fluorophore and G bases, leading to a short fluorescence lifetime. Upon the folding of the motif to form a stable G-quadruplex structure, the intramolecular interactions and the concomitant PET could be eliminated with an increased fluorescence lifetime, leading to a large reduction in the FA response. On the basis of this mechanism, a novel, specific, and sensitive FA approach was developed for the detection of biologically and functionally important G-quadruplex structures. The approach is examined and validated using one normal G-quadruplex motif, five mutants, and six small cations and is potentially applicable to the study of G-quadruplexes at single molecule level, ligand screening, profiling of highly ordered DNA nanostructures, and biosensing.
关键词[WOS]: CIRCULAR-DICHROISM ;  POTASSIUM-ION ;  DNA APTAMER ;  MOLECULAR-BIOLOGY ;  HUMAN THROMBIN ;  POLARIZATION ;  STABILITY ;  BASE ;  OLIGODEOXYRIBONUCLEOTIDES ;  NANOPARTICLES
语种: 英语
WOS记录号: WOS:000308829700073
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118448
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
3.Shanxi Univ, Res Ctr Environm Sci & Engn, Taiyuan 030006, Shanxi Province, Peoples R China
4.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China

Recommended Citation:
Zhang, Dapeng,Shen, Hujun,Li, Guohui,et al. Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism[J]. ANALYTICAL CHEMISTRY,2012,84(18):8088-8094.
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