DICP OpenIR
Subject Area物理化学
Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism
Zhang, Dapeng1; Shen, Hujun2; Li, Guohui2; Zhao, Bailin1; Yu, Anchi4; Zhao, Qiang3; Wang, Hailin1; WangHailin
Source PublicationANALYTICAL CHEMISTRY
2012-09-18
ISSN0003-2700
DOI10.1021/ac302320x
Volume84Issue:18Pages:8088-8094
Indexed BySCI
SubtypeArticle
Department11
Funding Project1106
Contribution Rank2,2
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordCIRCULAR-DICHROISM ; POTASSIUM-ION ; DNA APTAMER ; MOLECULAR-BIOLOGY ; HUMAN THROMBIN ; POLARIZATION ; STABILITY ; BASE ; OLIGODEOXYRIBONUCLEOTIDES ; NANOPARTICLES
AbstractFluorescence anisotropy (FA) is a homogeneous, ratiometric, and real-time analytical technology. By selective labeling of a guanine (G)-quadruplex motif with tetramethylrhodamine (TMR), here, it is established that a large reduction in FA response can be specifically associated with the unfolding -> folding transition of G-quadruplex structures. On the basis of fluorescence intensity, polarization and lifetime analysis, and molecular docking simulation, the mechanism was found to be that the labeled fluorophore (TMR) can intramolecularly interact with adjacent G bases in an unfolded G-quadruplex motif, which allows for the photoinduced electron transfer (PET) occurring between the fluorophore and G bases, leading to a short fluorescence lifetime. Upon the folding of the motif to form a stable G-quadruplex structure, the intramolecular interactions and the concomitant PET could be eliminated with an increased fluorescence lifetime, leading to a large reduction in the FA response. On the basis of this mechanism, a novel, specific, and sensitive FA approach was developed for the detection of biologically and functionally important G-quadruplex structures. The approach is examined and validated using one normal G-quadruplex motif, five mutants, and six small cations and is potentially applicable to the study of G-quadruplexes at single molecule level, ligand screening, profiling of highly ordered DNA nanostructures, and biosensing.
Language英语
WOS IDWOS:000308829700073
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118448
Collection中国科学院大连化学物理研究所
Corresponding AuthorWangHailin
Affiliation1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
3.Shanxi Univ, Res Ctr Environm Sci & Engn, Taiyuan 030006, Shanxi Province, Peoples R China
4.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Dapeng,Shen, Hujun,Li, Guohui,et al. Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism[J]. ANALYTICAL CHEMISTRY,2012,84(18):8088-8094.
APA Zhang, Dapeng.,Shen, Hujun.,Li, Guohui.,Zhao, Bailin.,Yu, Anchi.,...&WangHailin.(2012).Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism.ANALYTICAL CHEMISTRY,84(18),8088-8094.
MLA Zhang, Dapeng,et al."Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism".ANALYTICAL CHEMISTRY 84.18(2012):8088-8094.
Files in This Item:
File Name/Size DocType Version Access License
20121bn9sVWXXZ.PDF(392KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Dapeng]'s Articles
[Shen, Hujun]'s Articles
[Li, Guohui]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Dapeng]'s Articles
[Shen, Hujun]'s Articles
[Li, Guohui]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Dapeng]'s Articles
[Shen, Hujun]'s Articles
[Li, Guohui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.