DICP OpenIR
Subject Area物理化学
Enhanced top-down characterization of histone post-translational modifications
Tian, Zhixin1,2; Tolic, Nikola1; Zhao, Rui1; Moore, Ronald J.3; Hengel, Shawna M.1; Robinson, Errol W.1; Stenoien, David L.3; Wu, Si1; Smith, Richard D.3; Pasa-Tolic, Ljiljana1; LjiljanaPaš; aTolić
KeywordSaltless Wcx-hilic Top-down Histone Posttranslational Modification
Source PublicationGENOME BIOLOGY
2012
DOI10.1186/gb-2012-13-10-R86
Volume13Issue:10Pages:1
Indexed BySCI
SubtypeArticle
DepartmentDNL20
Funding Project2003
Contribution Rank2,1
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS KeywordMASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; H4 ; ISOFORMS ; REPAIR ; DOMAIN ; CODE ; H3
AbstractPost-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 mu g purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms.
Language英语
WOS IDWOS:000313183900005
Citation statistics
Cited Times:83[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/118454
Collection中国科学院大连化学物理研究所
Corresponding AuthorLjiljanaPaš; aTolić
Affiliation1.Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
3.Pacific NW Natl Lab, Biol Sci Div, Richland, WA 99352 USA
Recommended Citation
GB/T 7714
Tian, Zhixin,Tolic, Nikola,Zhao, Rui,et al. Enhanced top-down characterization of histone post-translational modifications[J]. GENOME BIOLOGY,2012,13(10):1.
APA Tian, Zhixin.,Tolic, Nikola.,Zhao, Rui.,Moore, Ronald J..,Hengel, Shawna M..,...&aTolić.(2012).Enhanced top-down characterization of histone post-translational modifications.GENOME BIOLOGY,13(10),1.
MLA Tian, Zhixin,et al."Enhanced top-down characterization of histone post-translational modifications".GENOME BIOLOGY 13.10(2012):1.
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