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学科主题: 物理化学
题名: Enhanced top-down characterization of histone post-translational modifications
作者: Tian, Zhixin1, 2;  Tolic, Nikola1;  Zhao, Rui1;  Moore, Ronald J.3;  Hengel, Shawna M.1;  Robinson, Errol W.1;  Stenoien, David L.3;  Wu, Si1;  Smith, Richard D.3;  Pasa-Tolic, Ljiljana1
通讯作者: LjiljanaPa&#353 ;  aToli&#263
关键词: Saltless WCX-HILIC ;  top-down ;  histone ;  posttranslational modification
刊名: GENOME BIOLOGY
发表日期: 2012
DOI: 10.1186/gb-2012-13-10-R86
卷: 13, 期:10, 页:1
收录类别: SCI
文章类型: Article
部门归属: DNL20
项目归属: 2003
产权排名: 2,1
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Genetics & Heredity
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Genetics & Heredity
英文摘要: Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 mu g purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms.
关键词[WOS]: MASS-SPECTROMETRY ;  LIQUID-CHROMATOGRAPHY ;  H4 ;  ISOFORMS ;  REPAIR ;  DOMAIN ;  CODE ;  H3
语种: 英语
WOS记录号: WOS:000313183900005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/118454
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
3.Pacific NW Natl Lab, Biol Sci Div, Richland, WA 99352 USA

Recommended Citation:
Tian, Zhixin,Tolic, Nikola,Zhao, Rui,et al. Enhanced top-down characterization of histone post-translational modifications[J]. GENOME BIOLOGY,2012,13(10):1.
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