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学科主题物理化学
Enhanced top-down characterization of histone post-translational modifications
Tian, Zhixin1,2; Tolic, Nikola1; Zhao, Rui1; Moore, Ronald J.3; Hengel, Shawna M.1; Robinson, Errol W.1; Stenoien, David L.3; Wu, Si1; Smith, Richard D.3; Pasa-Tolic, Ljiljana1; LjiljanaPaš; aTolić
关键词Saltless Wcx-hilic Top-down Histone Posttranslational Modification
刊名GENOME BIOLOGY
2012
DOI10.1186/gb-2012-13-10-R86
13期:10页:1
收录类别SCI
文章类型Article
部门归属DNL20
项目归属2003
产权排名2,1
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; H4 ; ISOFORMS ; REPAIR ; DOMAIN ; CODE ; H3
英文摘要Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 mu g purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms.
语种英语
WOS记录号WOS:000313183900005
引用统计
被引频次:75[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/118454
专题中国科学院大连化学物理研究所
通讯作者LjiljanaPaš; aTolić
作者单位1.Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
3.Pacific NW Natl Lab, Biol Sci Div, Richland, WA 99352 USA
推荐引用方式
GB/T 7714
Tian, Zhixin,Tolic, Nikola,Zhao, Rui,et al. Enhanced top-down characterization of histone post-translational modifications[J]. GENOME BIOLOGY,2012,13(10):1.
APA Tian, Zhixin.,Tolic, Nikola.,Zhao, Rui.,Moore, Ronald J..,Hengel, Shawna M..,...&aTolić.(2012).Enhanced top-down characterization of histone post-translational modifications.GENOME BIOLOGY,13(10),1.
MLA Tian, Zhixin,et al."Enhanced top-down characterization of histone post-translational modifications".GENOME BIOLOGY 13.10(2012):1.
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