中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds
作者: Zhong, Weiliang1;  Zhang, Weiguo1;  Wang, Shouyu1;  Qin, Jianhua2
通讯作者: Weiguo Zhang
刊名: PLOS ONE
发表日期: 2013-04-18
DOI: 10.1371/journal.pone.0061283
卷: 8, 期:4, 页:61283
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 待补充
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
资助者: 2,4
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: In native fibrocartilage, mechanotransduction allows the cells to perceive the physical microenvironment not only through topographical cues from the extracellular matrix, but also through mechanical cues, such as interstitial flow. To create a microenvironment that simultaneously integrates nanotopography and flow stimulus, we developed a biomimetic microfluidic device embedded with aligned nanofibers to contain microchambers of different angles, which enabled the flow direction to form different angles with the fibers. Using this device, we investigated the effects of microfluidic and nanotopographical environment on the morphology and fibrochondrogenesis of mesenchymal stem cells (MSCs) and the involvement of RhoA/ROCK pathway and Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ). The results showed that the flow direction perpendicular to aligned nanofibers was conducive to fibrochondrogenesis of MSCs. In addition, ROCK inhibitor and knockdown of YAP/TAZ disrupted fibrochondrogenic differentiation of MSCs. In conclusion, our data suggest the crucial role of mechanotransduction in regulating fibrochondrogenic differentiation of MSCs, which may be mediated by RhoA/ROCK pathway and YAP/TAZ.
关键词[WOS]: GENE-EXPRESSION ;  CHONDROGENIC DIFFERENTIATION ;  FOCAL ADHESION ;  NUCLEAR SHAPE ;  FATE ;  MICROENVIRONMENT ;  MODULATION ;  PATHWAY ;  REGENERATION ;  CHONDROCYTES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000317908700019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119086
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
20130svqfJZmS6.PDF(2265KB)----开放获取View Download

作者单位: 1.Dalian Med Univ, Affiliated Hosp 1, Dept Orthopaed, Dalian, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China

Recommended Citation:
Zhong, Weiliang,Zhang, Weiguo,Wang, Shouyu,et al. Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds[J]. PLOS ONE,2013,8(4):61283.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Zhong, Weiliang]'s Articles
 [Zhang, Weiguo]'s Articles
 [Wang, Shouyu]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Zhong, Weiliang]‘s Articles
 [Zhang, Weiguo]‘s Articles
 [Wang, Shouyu]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 20130svqfJZmS6.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace