DICP OpenIR
学科主题物理化学
Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique
Li, Qinran1,2; Yang, Kaiguang1; Liu, Jinxiang1,2; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Hierarchical Imprinting Protein Imprinted Polymers Transferrin. Selective Recognition
刊名MICROCHIMICA ACTA
2013-11-01
DOI10.1007/s00604-013-0994-7
180期:15-16页:1379-1386
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1810
产权排名待补充
WOS标题词Science & Technology ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]ELECTROSTATIC FUNCTIONAL-GROUPS ; ENZYME-INHIBITORS ; RIBONUCLEASE-A ; POLYMERS ; POLYACRYLAMIDE ; PLASMA ; PROTEOMICS ; HYDROGEL ; BEADS ; SDS
英文摘要A novel kind of transferrin imprinted polymer particles was synthesized by a hierarchical strategy. First, transferrin was immobilized on silica beads by non-covalent absorption. Then, a pre-polymerization mixture, composed of 1,4-bis(acryloyl)piperazine, methacrylamide, methacrylic acid, ammonium sulfate and polyoxyethylene sorbitan monolaurate, was irrigated into the pores of silica particles, and polymerized at 25 A degrees C. Finally, the silica matrix was etched with ammonium hydrogen fluoride, not only to remove the template protein, but also to expose protein recognition sites on the surface of the imprinted polymer. The binding capacity of the transferrin-imprinted particles is 6.3 mg of protein per gram of material, and the time required to reach adsorption equilibrium was less than 10 min. The imprinting factor of transferrin is ca. 3.3 in the presence of ribonuclease B, cytochrome c and beta-lactoglobulin. The results indicate that these imprinted polymer particles can recognize transferrin with good selectivity, high binding capacity and fast mass transfer. They may be applied as an artificial antibody to remove the high abundance proteins in plasma.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
原文出处查看原文
WOS记录号WOS:000326803800004
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119109
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Li, Qinran,Yang, Kaiguang,Liu, Jinxiang,et al. Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique[J]. MICROCHIMICA ACTA,2013,180(15-16):1379-1386.
APA Li, Qinran.,Yang, Kaiguang.,Liu, Jinxiang.,Zhang, Lihua.,Liang, Zhen.,...&张丽华.(2013).Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique.MICROCHIMICA ACTA,180(15-16),1379-1386.
MLA Li, Qinran,et al."Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique".MICROCHIMICA ACTA 180.15-16(2013):1379-1386.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
20133XHsM0prq2.PDF(364KB) 开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Qinran]的文章
[Yang, Kaiguang]的文章
[Liu, Jinxiang]的文章
百度学术
百度学术中相似的文章
[Li, Qinran]的文章
[Yang, Kaiguang]的文章
[Liu, Jinxiang]的文章
必应学术
必应学术中相似的文章
[Li, Qinran]的文章
[Yang, Kaiguang]的文章
[Liu, Jinxiang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。