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学科主题: 物理化学
题名: Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique
作者: Li, Qinran1, 2;  Yang, Kaiguang1;  Liu, Jinxiang1, 2;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Hierarchical imprinting ;  Protein imprinted polymers ;  Transferrin. Selective recognition
刊名: MICROCHIMICA ACTA
发表日期: 2013-11-01
DOI: 10.1007/s00604-013-0994-7
卷: 180, 期:15-16, 页:1379-1386
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Chemistry
英文摘要: A novel kind of transferrin imprinted polymer particles was synthesized by a hierarchical strategy. First, transferrin was immobilized on silica beads by non-covalent absorption. Then, a pre-polymerization mixture, composed of 1,4-bis(acryloyl)piperazine, methacrylamide, methacrylic acid, ammonium sulfate and polyoxyethylene sorbitan monolaurate, was irrigated into the pores of silica particles, and polymerized at 25 A degrees C. Finally, the silica matrix was etched with ammonium hydrogen fluoride, not only to remove the template protein, but also to expose protein recognition sites on the surface of the imprinted polymer. The binding capacity of the transferrin-imprinted particles is 6.3 mg of protein per gram of material, and the time required to reach adsorption equilibrium was less than 10 min. The imprinting factor of transferrin is ca. 3.3 in the presence of ribonuclease B, cytochrome c and beta-lactoglobulin. The results indicate that these imprinted polymer particles can recognize transferrin with good selectivity, high binding capacity and fast mass transfer. They may be applied as an artificial antibody to remove the high abundance proteins in plasma.
关键词[WOS]: ELECTROSTATIC FUNCTIONAL-GROUPS ;  ENZYME-INHIBITORS ;  RIBONUCLEASE-A ;  POLYMERS ;  POLYACRYLAMIDE ;  PLASMA ;  PROTEOMICS ;  HYDROGEL ;  BEADS ;  SDS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000326803800004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119109
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Li, Qinran,Yang, Kaiguang,Liu, Jinxiang,et al. Transferrin recognition based on a protein imprinted material prepared by hierarchical imprinting technique[J]. MICROCHIMICA ACTA,2013,180(15-16):1379-1386.
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