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学科主题: 物理化学
题名: Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns
作者: Liu, Zheyi1;  Ou, Junjie1;  Liu, Zhongshan1, 2;  Liu, Jing1, 2;  Lin, Hui1, 2;  Wang, Fangjun1;  Zou, Hanfa1
通讯作者: 王方军 ;  邹汉法
关键词: Hybrid monolithic capillary column ;  Intact protein ;  Reversed phase liquid chromatography ;  High speed separation
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2013-11-22
DOI: 10.1016/j.chroma.2013.09.004
卷: 1317, 页:138-147
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: High-efficient separation of intact proteins is still a huge challenge in proteome analysis of complex biological samples by using capillary columns. In this study, four FOSS-based hybrid monolithic capillary columns were prepared and applied in nano-flow liquid chromatography (Nano-LC) separation of intact proteins. It was observed that the POSS-based hybrid monolithic columns exhibit high permeability, good LC separation reproducibility and column efficiency for intact protein separation. The effects of different LC separation conditions such as flow rate, gradient steepness, column length and mobile phase additives on the LC separation efficiency of the POSS-based hybrid monolithic column were systematically examined. Finally, fast LC separation of 7 proteins mixture was realized in 2.5 min by using the optimized conditions on the 100 mu m i.d. POSS-based hybrid monolithic capillary column. (C) 2013 Published by Elsevier B.V.
关键词[WOS]: LIQUID-CHROMATOGRAPHIC SEPARATION ;  SPECTROMETRY-BASED PROTEOMICS ;  TOP-DOWN PROTEOMICS ;  ON-PLATE DIGESTION ;  MASS-SPECTROMETRY ;  QUANTITATIVE-ANALYSIS ;  STATIONARY PHASES ;  GRADIENT-ELUTION ;  PEAK-CAPACITY ;  IDENTIFICATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000327229600016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119129
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Liu, Zheyi,Ou, Junjie,Liu, Zhongshan,et al. Separation of intact proteins by using polyhedral oligomeric silsesquioxane based hybrid monolithic capillary columns[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1317:138-147.
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