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学科主题物理化学
High throughput generation and trapping of individual agarose microgel using microfluidic approach
Shi, Yang1; Gao, Xinghua1; Chen, Longqing2; Zhang, Min1; Ma, Jingyun1; Zhang, Xixiang2; Qin, Jianhua1; Qin JH(秦建华)
关键词Agarose Microgel 3d Cell Culture Microfluidic Droplet
刊名MICROFLUIDICS AND NANOFLUIDICS
2013-10-01
ISSN1613-4982
DOI10.1007/s10404-013-1160-6
15期:4页:467-474
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1807
产权排名待补充
WOS标题词Science & Technology ; Technology ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Nanoscience & Nanotechnology ; Instruments & Instrumentation ; Physics, Fluids & Plasmas
研究领域[WOS]Science & Technology - Other Topics ; Instruments & Instrumentation ; Physics
关键词[WOS]DRUG-DELIVERY ; MICROPARTICLES ; PARTICLES ; CELLS ; FLOW ; GEL
英文摘要Microgel is a kind of biocompatible polymeric material, which has been widely used as micro-carriers in materials synthesis, drug delivery and cell biology applications. However, high-throughput generation of individual microgel for on-site analysis in a microdevice still remains a challenge. Here, we presented a simple and stable droplet microfluidic system to realize high-throughput generation and trapping of individual agarose microgels based on the synergetic effect of surface tension and hydrodynamic forces in microchannels and used it for 3-D cell culture in real-time. The established system was mainly composed of droplet generators with flow focusing T-junction and a series of array individual trap structures. The whole process including the independent agarose microgel formation, immobilization in trapping array and gelation in situ via temperature cooling could be realized on the integrated microdevice completely. The performance of this system was demonstrated by successfully encapsulating and culturing adenoid cystic carcinoma (ACCM) cells in the gelated agarose microgels. This established approach is simple, easy to operate, which can not only generate the micro-carriers with different components in parallel, but also monitor the cell behavior in 3D matrix in real-time. It can also be extended for applications in the area of material synthesis and tissue engineering.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
原文出处查看原文
WOS记录号WOS:000324646000003
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119167
专题中国科学院大连化学物理研究所
通讯作者Qin JH(秦建华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.King Abdullah Univ Sci & Technol, Adv Nanofabricat Imaging & Characterizat Core Lab, Thuwal 239556900, Saudi Arabia
推荐引用方式
GB/T 7714
Shi, Yang,Gao, Xinghua,Chen, Longqing,et al. High throughput generation and trapping of individual agarose microgel using microfluidic approach[J]. MICROFLUIDICS AND NANOFLUIDICS,2013,15(4):467-474.
APA Shi, Yang.,Gao, Xinghua.,Chen, Longqing.,Zhang, Min.,Ma, Jingyun.,...&秦建华.(2013).High throughput generation and trapping of individual agarose microgel using microfluidic approach.MICROFLUIDICS AND NANOFLUIDICS,15(4),467-474.
MLA Shi, Yang,et al."High throughput generation and trapping of individual agarose microgel using microfluidic approach".MICROFLUIDICS AND NANOFLUIDICS 15.4(2013):467-474.
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