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学科主题: 物理化学
题名: High throughput generation and trapping of individual agarose microgel using microfluidic approach
作者: Shi, Yang1;  Gao, Xinghua1;  Chen, Longqing2;  Zhang, Min1;  Ma, Jingyun1;  Zhang, Xixiang2;  Qin, Jianhua1
通讯作者: 秦建华
关键词: Agarose ;  Microgel ;  3D cell culture ;  Microfluidic ;  Droplet
刊名: MICROFLUIDICS AND NANOFLUIDICS
发表日期: 2013-10-01
DOI: 10.1007/s10404-013-1160-6
卷: 15, 期:4, 页:467-474
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 待补充
WOS标题词: Science & Technology ;  Technology ;  Physical Sciences
类目[WOS]: Nanoscience & Nanotechnology ;  Instruments & Instrumentation ;  Physics, Fluids & Plasmas
资助者: 1,1
研究领域[WOS]: Science & Technology - Other Topics ;  Instruments & Instrumentation ;  Physics
英文摘要: Microgel is a kind of biocompatible polymeric material, which has been widely used as micro-carriers in materials synthesis, drug delivery and cell biology applications. However, high-throughput generation of individual microgel for on-site analysis in a microdevice still remains a challenge. Here, we presented a simple and stable droplet microfluidic system to realize high-throughput generation and trapping of individual agarose microgels based on the synergetic effect of surface tension and hydrodynamic forces in microchannels and used it for 3-D cell culture in real-time. The established system was mainly composed of droplet generators with flow focusing T-junction and a series of array individual trap structures. The whole process including the independent agarose microgel formation, immobilization in trapping array and gelation in situ via temperature cooling could be realized on the integrated microdevice completely. The performance of this system was demonstrated by successfully encapsulating and culturing adenoid cystic carcinoma (ACCM) cells in the gelated agarose microgels. This established approach is simple, easy to operate, which can not only generate the micro-carriers with different components in parallel, but also monitor the cell behavior in 3D matrix in real-time. It can also be extended for applications in the area of material synthesis and tissue engineering.
关键词[WOS]: DRUG-DELIVERY ;  MICROPARTICLES ;  PARTICLES ;  CELLS ;  FLOW ;  GEL
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000324646000003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119167
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.King Abdullah Univ Sci & Technol, Adv Nanofabricat Imaging & Characterizat Core Lab, Thuwal 239556900, Saudi Arabia

Recommended Citation:
Shi, Yang,Gao, Xinghua,Chen, Longqing,et al. High throughput generation and trapping of individual agarose microgel using microfluidic approach[J]. MICROFLUIDICS AND NANOFLUIDICS,2013,15(4):467-474.
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