DICP OpenIR
Subject Area物理化学
High throughput generation and trapping of individual agarose microgel using microfluidic approach
Shi, Yang1; Gao, Xinghua1; Chen, Longqing2; Zhang, Min1; Ma, Jingyun1; Zhang, Xixiang2; Qin, Jianhua1; Qin JH(秦建华)
KeywordAgarose Microgel 3d Cell Culture Microfluidic Droplet
Source PublicationMICROFLUIDICS AND NANOFLUIDICS
2013-10-01
ISSN1613-4982
DOI10.1007/s10404-013-1160-6
Volume15Issue:4Pages:467-474
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1807
Contribution Rank待补充
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectNanoscience & Nanotechnology ; Instruments & Instrumentation ; Physics, Fluids & Plasmas
WOS Research AreaScience & Technology - Other Topics ; Instruments & Instrumentation ; Physics
WOS KeywordDRUG-DELIVERY ; MICROPARTICLES ; PARTICLES ; CELLS ; FLOW ; GEL
AbstractMicrogel is a kind of biocompatible polymeric material, which has been widely used as micro-carriers in materials synthesis, drug delivery and cell biology applications. However, high-throughput generation of individual microgel for on-site analysis in a microdevice still remains a challenge. Here, we presented a simple and stable droplet microfluidic system to realize high-throughput generation and trapping of individual agarose microgels based on the synergetic effect of surface tension and hydrodynamic forces in microchannels and used it for 3-D cell culture in real-time. The established system was mainly composed of droplet generators with flow focusing T-junction and a series of array individual trap structures. The whole process including the independent agarose microgel formation, immobilization in trapping array and gelation in situ via temperature cooling could be realized on the integrated microdevice completely. The performance of this system was demonstrated by successfully encapsulating and culturing adenoid cystic carcinoma (ACCM) cells in the gelated agarose microgels. This established approach is simple, easy to operate, which can not only generate the micro-carriers with different components in parallel, but also monitor the cell behavior in 3D matrix in real-time. It can also be extended for applications in the area of material synthesis and tissue engineering.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
URL查看原文
WOS IDWOS:000324646000003
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119167
Collection中国科学院大连化学物理研究所
Corresponding AuthorQin JH(秦建华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.King Abdullah Univ Sci & Technol, Adv Nanofabricat Imaging & Characterizat Core Lab, Thuwal 239556900, Saudi Arabia
Recommended Citation
GB/T 7714
Shi, Yang,Gao, Xinghua,Chen, Longqing,et al. High throughput generation and trapping of individual agarose microgel using microfluidic approach[J]. MICROFLUIDICS AND NANOFLUIDICS,2013,15(4):467-474.
APA Shi, Yang.,Gao, Xinghua.,Chen, Longqing.,Zhang, Min.,Ma, Jingyun.,...&秦建华.(2013).High throughput generation and trapping of individual agarose microgel using microfluidic approach.MICROFLUIDICS AND NANOFLUIDICS,15(4),467-474.
MLA Shi, Yang,et al."High throughput generation and trapping of individual agarose microgel using microfluidic approach".MICROFLUIDICS AND NANOFLUIDICS 15.4(2013):467-474.
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