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学科主题: 物理化学
题名: Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings
作者: Yuan, Yue1;  Zhang, Jia3;  Wang, Mengjing1;  Mei, Bin1;  Guan, Yafeng2;  Liang, Gaolin1
通讯作者: Gaolin Liang
刊名: ANALYTICAL CHEMISTRY
发表日期: 2013-02-05
DOI: 10.1021/ac303183v
卷: 85, 期:3, 页:1280-1284
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 1
项目归属: 105
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 2,5
研究领域[WOS]: Chemistry
英文摘要: Taking advantage of a reduction-controlled biocompatible condensation reaction and self-assembly, we have developed a new method for the determination of glutathione (GSH) concentration in vitro and in HepG(2) human liver cancer cells. Upon reduction by GSH under physiological conditions (pH 7.4 in buffer), the small molecule CBT-Cys(SEt) condenses and self-assembles into nanorings, increasing the UV absorbance at 380 nm (with significant linear correlation in the 0-87 mu M GSH range and a limit of detection of 1 mu M). This method is also selective to GSH rather than cysteine in biological samples. Through the use of added internal standards, we successfully determined the concentration of GSH in HepG2 cells to be 14.96 mu M (2.99 fmol/cell). To better understand the mechanism of nanoring self-assembly, the condensation product of CBT-Cys(SEt) formed using different concentrations of GSH and different reaction times were characterized by transmission electron microscopy (TEM).
关键词[WOS]: BIOCOMPATIBLE CONDENSATION REACTION ;  THIOLS ;  DISULFIDES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000314676100006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119168
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Sci & Technol China, Dept Chem, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

Recommended Citation:
Yuan, Yue,Zhang, Jia,Wang, Mengjing,et al. Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings[J]. ANALYTICAL CHEMISTRY,2013,85(3):1280-1284.
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