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学科主题物理化学
Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings
Yuan, Yue1; Zhang, Jia3; Wang, Mengjing1; Mei, Bin1; Guan, Yafeng2; Liang, Gaolin1; Gaolin Liang
刊名ANALYTICAL CHEMISTRY
2013-02-05
DOI10.1021/ac303183v
85期:3页:1280-1284
收录类别SCI
合作性质
文章类型Article
部门归属1
项目归属105
产权排名待补充
WOS标题词Science & Technology ; Physical Sciences
资助者2,5 ; 2,5 ; 2,5 ; 2,5
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]BIOCOMPATIBLE CONDENSATION REACTION ; THIOLS ; DISULFIDES
英文摘要Taking advantage of a reduction-controlled biocompatible condensation reaction and self-assembly, we have developed a new method for the determination of glutathione (GSH) concentration in vitro and in HepG(2) human liver cancer cells. Upon reduction by GSH under physiological conditions (pH 7.4 in buffer), the small molecule CBT-Cys(SEt) condenses and self-assembles into nanorings, increasing the UV absorbance at 380 nm (with significant linear correlation in the 0-87 mu M GSH range and a limit of detection of 1 mu M). This method is also selective to GSH rather than cysteine in biological samples. Through the use of added internal standards, we successfully determined the concentration of GSH in HepG2 cells to be 14.96 mu M (2.99 fmol/cell). To better understand the mechanism of nanoring self-assembly, the condensation product of CBT-Cys(SEt) formed using different concentrations of GSH and different reaction times were characterized by transmission electron microscopy (TEM).
语种英语
资助者2,5 ; 2,5 ; 2,5 ; 2,5
原文出处查看原文
WOS记录号WOS:000314676100006
引用统计
被引频次:45[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119168
专题中国科学院大连化学物理研究所
通讯作者Gaolin Liang
作者单位1.Univ Sci & Technol China, Dept Chem, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China
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GB/T 7714
Yuan, Yue,Zhang, Jia,Wang, Mengjing,et al. Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings[J]. ANALYTICAL CHEMISTRY,2013,85(3):1280-1284.
APA Yuan, Yue.,Zhang, Jia.,Wang, Mengjing.,Mei, Bin.,Guan, Yafeng.,...&Gaolin Liang.(2013).Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings.ANALYTICAL CHEMISTRY,85(3),1280-1284.
MLA Yuan, Yue,et al."Detection of Glutathione in Vitro and in Cells by the Controlled Self-Assembly of Nanorings".ANALYTICAL CHEMISTRY 85.3(2013):1280-1284.
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