DICP OpenIR
Subject Area物理化学
Improvement of mannitol production by Lactobacillus brevis mutant 3-A5 based on dual-stage pH control and fed-batch fermentations
Yue, Min1,2; Cao, Hailong1; Zhang, Jianping1,2; Li, Shuguang1; Meng, Yanyu1,2; Chen, Wei1; Huang, Lishuxin1,2; Du, Yuguang1; Du YG(杜昱光)
KeywordMannitol Dual-stage Ph Control Lactobacillus Brevis Batch Fermentation Fed-batch Fermentation
Source PublicationWORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
2013-10-01
ISSN0959-3993
DOI10.1007/s11274-013-1357-6
Volume29Issue:10Pages:1923-1930
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1805
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectBiotechnology & Applied Microbiology
WOS Research AreaBiotechnology & Applied Microbiology
WOS KeywordINTERMEDIUS NRRL B-3693 ; LACTIC-ACID BACTERIA ; REUTERI CRL 1101 ; ORGANIC-ACIDS ; GROWTH
AbstractLactobacillus brevis 3-A5 was isolated and expected to produce mannitol efficiently by regulating pH in batch and fed-batch fermentations. In 48 h batch fermentations with free and constant pH, the optimal pH for cell growth and mannitol production in the first 24 h of incubation was 5.5, whereas that for mannitol production in the second 24 h of incubation was 4.5. To achieve high cell density and mannitol yield simultaneously, a dual-stage pH control strategy was proposed based on the kinetic analysis of mannitol production. The pH value was controlled at 5.5 for the first 12 h of fermentation and subsequently shifted to 4.5 until the fermentation was completed. Under dual-stage pH control fermentation, a 103 g/L yield of mannitol with a volumetric production rate of 3.7 g/L/h was achieved after 28 h. The dual-stage pH control fed-batch fermentation strategy was further developed to improve mannitol yield, wherein the yield increased by 109 % to 215 g/L after 98 h of fermentation. This value is the highest yield of mannitol ever reported using L. brevis.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
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WOS IDWOS:000325035100020
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119287
Collection中国科学院大连化学物理研究所
Corresponding AuthorDu YG(杜昱光)
Affiliation1.Chinese Acad Sci, Nat Prod & Glycobiotechnol Res Grp, Liaoning Prov Key Lab Carbohydrates, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yue, Min,Cao, Hailong,Zhang, Jianping,et al. Improvement of mannitol production by Lactobacillus brevis mutant 3-A5 based on dual-stage pH control and fed-batch fermentations[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2013,29(10):1923-1930.
APA Yue, Min.,Cao, Hailong.,Zhang, Jianping.,Li, Shuguang.,Meng, Yanyu.,...&杜昱光.(2013).Improvement of mannitol production by Lactobacillus brevis mutant 3-A5 based on dual-stage pH control and fed-batch fermentations.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,29(10),1923-1930.
MLA Yue, Min,et al."Improvement of mannitol production by Lactobacillus brevis mutant 3-A5 based on dual-stage pH control and fed-batch fermentations".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 29.10(2013):1923-1930.
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