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学科主题物理化学
An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells
Wang, Lei1,2,3; Zhou, Yongjin J.1,2; Ji, Debin1,2; Zhao, Zongbao K.1,2,3; Zhao ZB(赵宗保)
关键词Intracellular Aqueous Volume Nad(+) Escherichia Coil Metabolite Agrobacterium Tumefaciens
刊名JOURNAL OF MICROBIOLOGICAL METHODS
2013-05-01
DOI10.1016/j.mimet.2013.02.006
93期:2页:73-76
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1816
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Biochemical Research Methods ; Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Microbiology
关键词[WOS]METABOLITE CONCENTRATIONS ; WATER VOLUME ; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE ; QUANTIFICATION ; METABOLOMICS ; CEREVISIAE
英文摘要The intracellular aqueous volumes (V-in) of microorganisms are fundamental data that can be used for estimating absolute cellular enzyme and metabolite concentrations. Because traditional methods are time-consuming and costly, the V-in data have been largely estimated ambiguously. Here we developed an NAD(+) concentration-dependent method and demonstrated its usefulness for accurate estimation of the V-in value of Escherichia coil cells. The V-in value of E. coli BL21(DE3) cells was determined to be 1.9 mu L.mg(-1), which is 17% lower than that of the commonly assumed data. Similarly, the V-in value of Agrobacterium tumefaciens AGL1 cells was determined to be 1.8 mu L.mg(-1). Because NAD(+) is routinely quantified during metabolite analysis, it may be integrated into metabolomic data collection with little additional time and labor expenditure. This method should also be applicable to estimate the V-in data of other prokaryotic microorganisms. (C) 2013 Elsevier B.V. All rights reserved.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
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WOS记录号WOS:000318754600001
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119317
专题中国科学院大连化学物理研究所
通讯作者Zhao ZB(赵宗保)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wang, Lei,Zhou, Yongjin J.,Ji, Debin,et al. An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2013,93(2):73-76.
APA Wang, Lei,Zhou, Yongjin J.,Ji, Debin,Zhao, Zongbao K.,&赵宗保.(2013).An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells.JOURNAL OF MICROBIOLOGICAL METHODS,93(2),73-76.
MLA Wang, Lei,et al."An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells".JOURNAL OF MICROBIOLOGICAL METHODS 93.2(2013):73-76.
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