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学科主题: 物理化学
题名: An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells
作者: Wang, Lei1, 2, 3;  Zhou, Yongjin J.1, 2;  Ji, Debin1, 2;  Zhao, Zongbao K.1, 2, 3
通讯作者: 赵宗保
关键词: Intracellular aqueous volume ;  NAD(+) ;  Escherichia coil ;  Metabolite ;  Agrobacterium tumefaciens
刊名: JOURNAL OF MICROBIOLOGICAL METHODS
发表日期: 2013-05-01
DOI: 10.1016/j.mimet.2013.02.006
卷: 93, 期:2, 页:73-76
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Microbiology
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology ;  Microbiology
英文摘要: The intracellular aqueous volumes (V-in) of microorganisms are fundamental data that can be used for estimating absolute cellular enzyme and metabolite concentrations. Because traditional methods are time-consuming and costly, the V-in data have been largely estimated ambiguously. Here we developed an NAD(+) concentration-dependent method and demonstrated its usefulness for accurate estimation of the V-in value of Escherichia coil cells. The V-in value of E. coli BL21(DE3) cells was determined to be 1.9 mu L.mg(-1), which is 17% lower than that of the commonly assumed data. Similarly, the V-in value of Agrobacterium tumefaciens AGL1 cells was determined to be 1.8 mu L.mg(-1). Because NAD(+) is routinely quantified during metabolite analysis, it may be integrated into metabolomic data collection with little additional time and labor expenditure. This method should also be applicable to estimate the V-in data of other prokaryotic microorganisms. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: METABOLITE CONCENTRATIONS ;  WATER VOLUME ;  GLUCOSE-6-PHOSPHATE-DEHYDROGENASE ;  QUANTIFICATION ;  METABOLOMICS ;  CEREVISIAE
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000318754600001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119317
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Lei,Zhou, Yongjin J.,Ji, Debin,et al. An accurate method for estimation of the intracellular aqueous volume of Escherichia coli cells[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2013,93(2):73-76.
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