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学科主题物理化学
Enzyme Entrapped in Polymer-Modified Nanopores: The Effects of Macromolecular Crowding and Surface Hydrophobicity
Liu, Jia; Peng, Juan; Shen, Shuai; Jin, Qianru; Li, Can; Yang, Qihua; Li C(李灿); Yang QH(杨启华)
关键词Biocatalysis Immobilization Lipase Mesoporous Materials Nanopores
刊名CHEMISTRY-A EUROPEAN JOURNAL
2013-02-01
DOI10.1002/chem.201203833
19期:8页:2711-2719
收录类别SCI
合作性质
文章类型Article
部门归属5
项目归属506,503
产权排名待补充
WOS标题词Science & Technology ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]PROTEIN STABILITY ; INTERFACIAL ADSORPTION ; BIOCHEMICAL REACTIONS ; MESOPOROUS MATERIALS ; LIPASE ; CONFINEMENT ; IMMOBILIZATION ; SILICA ; BIOCATALYSIS ; HYBRID
英文摘要Macromolecular crowding is an ubiquitous phenomenon in living cells that significantly affects the function of enzymes invivo. However, this effect has not been paid much attention in the research of the immobilization of enzymes onto mesoporous silica. Herein, we report the combined effects of macromolecular crowding and surface hydrophobicity on the performance of an immobilized enzyme by accommodating lipase molecules into a series of mesoporous silicas with different amounts of inert poly(methacrylate) (PMA) covalently anchored inside the nanopores. The incorporation of the PMA polymer into the nanopores of mesoporous silica enables the fabrication of a crowded and hydrophobic microenvironment for the immobilized enzyme and the variation in polymer content facilitates an adjustment of the degree of crowding and surface properties of this environment. Based on this system, the catalytic features of immobilized lipase were investigated as a function of polymer content in nanopores and the results indicated that the catalytic efficiency, thermostability, and reusability of immobilized lipase could all be improved by taking advantage of the macromolecular crowding effect and surface hydrophobicity. These findings provide insight into the possible functions of the macromolecular crowding effect, which should be considered and integrated into the fabrication of suitable mesoporous silicas to improve enzyme immobilization.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
原文出处查看原文
WOS记录号WOS:000314926300020
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119330
专题中国科学院大连化学物理研究所
通讯作者Li C(李灿); Yang QH(杨启华)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Liu, Jia,Peng, Juan,Shen, Shuai,et al. Enzyme Entrapped in Polymer-Modified Nanopores: The Effects of Macromolecular Crowding and Surface Hydrophobicity[J]. CHEMISTRY-A EUROPEAN JOURNAL,2013,19(8):2711-2719.
APA Liu, Jia.,Peng, Juan.,Shen, Shuai.,Jin, Qianru.,Li, Can.,...&杨启华.(2013).Enzyme Entrapped in Polymer-Modified Nanopores: The Effects of Macromolecular Crowding and Surface Hydrophobicity.CHEMISTRY-A EUROPEAN JOURNAL,19(8),2711-2719.
MLA Liu, Jia,et al."Enzyme Entrapped in Polymer-Modified Nanopores: The Effects of Macromolecular Crowding and Surface Hydrophobicity".CHEMISTRY-A EUROPEAN JOURNAL 19.8(2013):2711-2719.
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