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学科主题: 物理化学
题名: Recent advances in monolithic columns for protein and peptide separation by capillary liquid chromatography
作者: Liang, Yu1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Monoliths ;  Capillary liquid chromatography ;  Proteins ;  Peptides ;  Separation
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2013-03-01
DOI: 10.1007/s00216-012-6570-x
卷: 405, 期:7, 页:2095-2106
收录类别: SCI
合作性质: 
文章类型: Review
部门归属: 18
项目归属: 1810
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Capillary liquid chromatography (cLC) has great potential for protein and peptide separation, with advantages of high efficiency, high resolution, low sample consumption, and high sensitivity when coupled with mass spectrometry. In recent years, monoliths have been widely used as the stationary phases for capillary columns, owing to easy preparation, high permeability, fast mass transfer, and low backpressure. This review summarizes recent advances (2007-2012) in monolithic columns for protein and peptide separation by cLC. After a brief introduction on the preparation of monolithic capillary columns, the emphasis of this review is focused on the recent application of such columns for protein and peptide separation by cLC. Furthermore, the challenges and potential hot points of monolithic capillary columns in the future are discussed.
关键词[WOS]: HYDROPHILIC INTERACTION CHROMATOGRAPHY ;  POROUS POLYMER MONOLITHS ;  ONE-POT APPROACH ;  MASS-SPECTROMETRY ;  SMALL MOLECULES ;  HIGH-EFFICIENCY ;  RADICAL POLYMERIZATION ;  OCTADECYL METHACRYLATE ;  GOLD NANOPARTICLES ;  PROTEOMICS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000315043800002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119334
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Liang, Yu,Zhang, Lihua,Zhang, Yukui. Recent advances in monolithic columns for protein and peptide separation by capillary liquid chromatography[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(7):2095-2106.
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