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学科主题: 物理化学
题名: Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins
作者: Yang, F.1;  Mao, J.1;  He, X. W.1;  Chen, L. X.1;  Zhang, Y. K.1, 2
通讯作者: 陈朗星 ;  张玉奎
关键词: Click chemistry ;  Boronate affinity hybrid monolith ;  Glycoproteins ;  Enrichment ;  Egg sample
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2013-06-01
DOI: 10.1007/s00216-013-6917-y
卷: 405, 期:15, 页:5321-5331
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
资助者: 2,5
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel strategy for preparation of a boronate affinity hybrid monolith was developed using a Cu(I)-catalyzed 1,3-dipolar azide-alkyne cycloaddition (CuAAC) reaction of an alkyne-boronate ligand with an azide-functionalized monolithic intermediate. An azide-functionalized hybrid monolith was first synthesized via a single-step procedure to provide reactive sites for click chemistry; then the alkyne-boronate ligands were covalently immobilized on the azide-functionalized hybrid monolith via an in-column CuAAC reaction to form a boronate affinity hybrid monolith under mild conditions. The boronate affinity monolith was characterized and evaluated by means of elemental analysis, Fourier transform infrared spectroscopy, and scanning electron microscopy. The boronate affinity hybrid monolith exhibited excellent specificity toward nucleosides and glycoproteins, which were chosen as test cis-diol-containing compounds under neutral conditions. The binding capacity of the monolith for the glycoprotein ovalbumin was 2.36 mg center dot g(-1) at pH 7.0. The practicability of the boronate affinity hybrid monolithic material was demonstrated by specific capture of the glycoproteins ovalbumin and ovotransferrin from an egg sample.
关键词[WOS]: CLICK CHEMISTRY ;  CAPILLARY CHROMATOGRAPHY ;  LIQUID-CHROMATOGRAPHY ;  SELECTIVE ENRICHMENT ;  EFFICIENT STRATEGY ;  MASS-SPECTROMETRY ;  STATIONARY PHASES ;  CIS-DIOL ;  COLUMN ;  SEPARATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000319168500029
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119341
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Yang, F.,Mao, J.,He, X. W.,et al. Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(15):5321-5331.
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