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学科主题物理化学
Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins
Yang, F.1; Mao, J.1; He, X. W.1; Chen, L. X.1; Zhang, Y. K.1,2; Chen LX(陈朗星); Zhang YK(张玉奎)
关键词Click Chemistry Boronate Affinity Hybrid Monolith Glycoproteins Enrichment Egg Sample
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2013-06-01
DOI10.1007/s00216-013-6917-y
405期:15页:5321-5331
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1810
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者2,5 ; 2,5 ; 2,5 ; 2,5
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CLICK CHEMISTRY ; CAPILLARY CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; SELECTIVE ENRICHMENT ; EFFICIENT STRATEGY ; MASS-SPECTROMETRY ; STATIONARY PHASES ; CIS-DIOL ; COLUMN ; SEPARATION
英文摘要A novel strategy for preparation of a boronate affinity hybrid monolith was developed using a Cu(I)-catalyzed 1,3-dipolar azide-alkyne cycloaddition (CuAAC) reaction of an alkyne-boronate ligand with an azide-functionalized monolithic intermediate. An azide-functionalized hybrid monolith was first synthesized via a single-step procedure to provide reactive sites for click chemistry; then the alkyne-boronate ligands were covalently immobilized on the azide-functionalized hybrid monolith via an in-column CuAAC reaction to form a boronate affinity hybrid monolith under mild conditions. The boronate affinity monolith was characterized and evaluated by means of elemental analysis, Fourier transform infrared spectroscopy, and scanning electron microscopy. The boronate affinity hybrid monolith exhibited excellent specificity toward nucleosides and glycoproteins, which were chosen as test cis-diol-containing compounds under neutral conditions. The binding capacity of the monolith for the glycoprotein ovalbumin was 2.36 mg center dot g(-1) at pH 7.0. The practicability of the boronate affinity hybrid monolithic material was demonstrated by specific capture of the glycoproteins ovalbumin and ovotransferrin from an egg sample.
语种英语
资助者2,5 ; 2,5 ; 2,5 ; 2,5
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WOS记录号WOS:000319168500029
引用统计
被引频次:44[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119341
专题中国科学院大连化学物理研究所
通讯作者Chen LX(陈朗星); Zhang YK(张玉奎)
作者单位1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Yang, F.,Mao, J.,He, X. W.,et al. Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(15):5321-5331.
APA Yang, F..,Mao, J..,He, X. W..,Chen, L. X..,Zhang, Y. K..,...&张玉奎.(2013).Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,405(15),5321-5331.
MLA Yang, F.,et al."Preparation of a boronate-functionalized affinity hybrid monolith for specific capture of glycoproteins".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 405.15(2013):5321-5331.
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