DICP OpenIR
Subject Area物理化学
FTO-mediated formation of N-6-hydroxymethyladenosine and N-6-formyladenosine in mammalian RNA
Fu, Ye1,2; Jia, Guifang1,2; Pang, Xueqin3,4,5,6; Wang, Richard N.1,2; Wang, Xiao1,2; Li, Charles J.1,2; Smemo, Scott7; Dai, Qing1,2; Bailey, Kathleen A.7; Nobrega, Marcelo A.7; Han, Ke-Li5,6; Cui, Qiang3,4; He, Chuan1,2; Ye Fu
Source PublicationNATURE COMMUNICATIONS
2013-04-01
DOI10.1038/ncomms2822
Volume4Pages:1798
Indexed BySCI
Cooperation Status
SubtypeArticle
Department11
Funding Project1101
Contribution Rank待补充
WOS HeadingsScience & Technology
Funding Organization3,3 ; 3,3 ; 3,3 ; 3,3
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordOBESITY-ASSOCIATED FTO ; MESSENGER-RNA ; ESCHERICHIA-COLI ; OXIDATIVE DEMETHYLATION ; ADULT OBESITY ; DNA ; GENE ; ALKB ; 5-HYDROXYMETHYLCYTOSINE ; 5-CARBOXYLCYTOSINE
AbstractN-6-methyladenosine is a prevalent internal modification in messenger RNA and non-coding RNA affecting various cellular pathways. Here we report the discovery of two additional modifications, N-6-hydroxymethyladenosine (hm(6)A) and N-6-formyladenosine (f(6)A), in mammalian messenger RNA. We show that Fe-II-and alpha-ketoglutarate-dependent fat mass and obesity-associated (FTO) protein oxidize N-6-methyladenosine to generate N-6-hydroxymethyladenosine as an intermediate modification, and N-6-formyladenosine as a further oxidized product. N-6-hydroxymethyladenosine and N-6-formyladenosine have half-life times of similar to 3 h in aqueous solution under physiological relevant conditions, and are present in isolated messenger RNA from human cells as well as mouse tissues. These previously unknown modifications derived from the prevalent N-6-methyladenosine in messenger RNA, formed through oxidative RNA demethylation, may dynamically modulate RNA-protein interactions to affect gene expression regulation.
Language英语
Funding Organization3,3 ; 3,3 ; 3,3 ; 3,3
URL查看原文
WOS IDWOS:000318872100155
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119390
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe Fu
Affiliation1.Univ Chicago, Dept Chem, Chicago, IL 60637 USA
2.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
3.Univ Wisconsin, Dept Chem, Madison, WI 53706 USA
4.Univ Wisconsin, Inst Theoret Chem, Madison, WI 53706 USA
5.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Liaoning, Peoples R China
6.Chinese Acad Sci, Dalian Inst Chem Phys, Ctr Theoret & Computat Chem, Dalian 116023, Liaoning, Peoples R China
7.Univ Chicago, Dept Human Genet, Chicago, IL 60637 USA
Recommended Citation
GB/T 7714
Fu, Ye,Jia, Guifang,Pang, Xueqin,et al. FTO-mediated formation of N-6-hydroxymethyladenosine and N-6-formyladenosine in mammalian RNA[J]. NATURE COMMUNICATIONS,2013,4:1798.
APA Fu, Ye.,Jia, Guifang.,Pang, Xueqin.,Wang, Richard N..,Wang, Xiao.,...&Ye Fu.(2013).FTO-mediated formation of N-6-hydroxymethyladenosine and N-6-formyladenosine in mammalian RNA.NATURE COMMUNICATIONS,4,1798.
MLA Fu, Ye,et al."FTO-mediated formation of N-6-hydroxymethyladenosine and N-6-formyladenosine in mammalian RNA".NATURE COMMUNICATIONS 4(2013):1798.
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