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学科主题: 物理化学
题名: Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein
作者: Gao, Feng-Xian1, 2;  Ma, Xiao-Tong1, 2;  He, Xi-Wen1, 2;  Li, Wen-You1, 2;  Zhang, Yu-Kui1, 2, 3
通讯作者: 李文友
关键词: Molecular imprinting ;  Surface imprinting ;  Covalent immobilization ;  Nanosphere ;  Ovalbumin
刊名: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
发表日期: 2013-09-20
DOI: 10.1016/j.colsurfa.2013.05.018
卷: 433, 期:20, 页:191-199
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Physical
资助者: 2,5
研究领域[WOS]: Chemistry
英文摘要: Through covalent immobilization of template and surface imprinting, smart molecularly imprinted polymer nanospheres were developed for selective separation of the glycoprotein ovalbumin (OB). First, a boronic acid group-bearing poly (methyl methacrylate) (b-PMMA) nanosphere was synthesized directly at the high temperature of 70 degrees C. The b-PMMA nanosphere could pre-immobilize the template OB on its surface by forming reversible covalent bonds. Then the precipitation polymerization of N-isopropylacrylamide (NIPAAm) and acrylamide (AAm) readily occurred on the b-PMMA nanosphere as a core at room temperature, leading to the formation of core-shell molecular imprinting nanosphere. The experiments showed the rebinding affinity of the imprinted nanospheres was thermo and pH-dependent. The resulting imprinted nanospheres showed high adsorption capacity and good specific recognition behavior toward the template molecule, and no obvious reusability deterioration was observed. Most notably, the imprinted nanospheres reached saturated adsorption within 20 min, indicating faster rebinding kinetics. In addition, the imprinted nanospheres were successfully applied to selectively separate the target OB from an egg white sample. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: PROTEIN RECOGNITION ;  MESOPOROUS SILICA ;  NANOPARTICLES ;  GLYCOPEPTIDES ;  ENRICHMENT ;  HYDROGELS ;  TEMPLATE ;  CAPTURE ;  CHROMATOGRAPHY ;  ANTIBODIES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000321538100023
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119454
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Gao, Feng-Xian,Ma, Xiao-Tong,He, Xi-Wen,et al. Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2013,433(20):191-199.
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