DICP OpenIR
Subject Area物理化学
Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein
Gao, Feng-Xian1,2; Ma, Xiao-Tong1,2; He, Xi-Wen1,2; Li, Wen-You1,2; Zhang, Yu-Kui1,2,3; Li WY(李文友)
KeywordMolecular Imprinting Surface Imprinting Covalent Immobilization Nanosphere Ovalbumin
Source PublicationCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
2013-09-20
DOI10.1016/j.colsurfa.2013.05.018
Volume433Issue:20Pages:191-199
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1810
Contribution Rank待补充
WOS HeadingsScience & Technology ; Physical Sciences
Funding Organization2,5 ; 2,5 ; 2,5 ; 2,5
WOS SubjectChemistry, Physical
WOS Research AreaChemistry
WOS KeywordPROTEIN RECOGNITION ; MESOPOROUS SILICA ; NANOPARTICLES ; GLYCOPEPTIDES ; ENRICHMENT ; HYDROGELS ; TEMPLATE ; CAPTURE ; CHROMATOGRAPHY ; ANTIBODIES
AbstractThrough covalent immobilization of template and surface imprinting, smart molecularly imprinted polymer nanospheres were developed for selective separation of the glycoprotein ovalbumin (OB). First, a boronic acid group-bearing poly (methyl methacrylate) (b-PMMA) nanosphere was synthesized directly at the high temperature of 70 degrees C. The b-PMMA nanosphere could pre-immobilize the template OB on its surface by forming reversible covalent bonds. Then the precipitation polymerization of N-isopropylacrylamide (NIPAAm) and acrylamide (AAm) readily occurred on the b-PMMA nanosphere as a core at room temperature, leading to the formation of core-shell molecular imprinting nanosphere. The experiments showed the rebinding affinity of the imprinted nanospheres was thermo and pH-dependent. The resulting imprinted nanospheres showed high adsorption capacity and good specific recognition behavior toward the template molecule, and no obvious reusability deterioration was observed. Most notably, the imprinted nanospheres reached saturated adsorption within 20 min, indicating faster rebinding kinetics. In addition, the imprinted nanospheres were successfully applied to selectively separate the target OB from an egg white sample. (C) 2013 Elsevier B.V. All rights reserved.
Language英语
Funding Organization2,5 ; 2,5 ; 2,5 ; 2,5
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WOS IDWOS:000321538100023
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119454
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi WY(李文友)
Affiliation1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Gao, Feng-Xian,Ma, Xiao-Tong,He, Xi-Wen,et al. Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2013,433(20):191-199.
APA Gao, Feng-Xian,Ma, Xiao-Tong,He, Xi-Wen,Li, Wen-You,Zhang, Yu-Kui,&李文友.(2013).Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,433(20),191-199.
MLA Gao, Feng-Xian,et al."Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 433.20(2013):191-199.
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