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学科主题: 物理化学
题名: Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites
作者: Li, Hua1;  Shan, Yuanhong1;  Qiao, Lizhen1;  Dou, Abo1;  Shi, Xianzhe1;  Xu, Guowang1
通讯作者: 石先哲 ;  许国旺
刊名: ANALYTICAL CHEMISTRY
发表日期: 2013-12-03
DOI: 10.1021/ac402979w
卷: 85, 期:23, 页:11585-11592
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Chemistry
英文摘要: Ribosylated metabolites, especially modified nucleosides, have been extensively evaluated as cancer-related biomarkers. Boronate adsorbents are considered to be promising materials for extracting them from complex matrices. However, the enrichment of ribosylated metabolites in low abundance is still a challenge due to the limited capacity and selectivity of the existing boronate adsorbents. In this study, a novel type of magnetic nanoparticles named Fe3O4@SiO2@PEI-FPBA was synthesized by grafting polyethyleneimine (PEI) onto the surface of Fe3O4@SiO2 before modification by boronate groups. The high density of the amino groups on the PEI chains supplied a large number of binding sites for boronate groups. Thus, the adsorption capacity (1.34 +/- 0.024 mg/g) of the nanoparticles, which is 6- to 7-fold higher than that of analogous materials, was greatly improved. The unreacted secondary amines and tertiary amines of the PEI enhanced the aqueous solubility of the nanoparticles, which could efficiently reduce nonspecific adsorption. The nanoparticles were able to capture 1,2 cis-diol nucleosides from 1000-fold interferences. Moreover, the flexible chains of PEI were favorable for effective enrichment and quick equilibration (<2 min). Finally, 60 ribose conjugates were enriched from human urine using the nanoparticles. Among them, 43 were identified to be nucleosides and other ribosylated metabolites. Nine low abundance modified nucleosides were detected for the first time. In conclusion, Fe3O4@SiO2@PEI-FPBA is an attractive candidate material for the highly selective enrichment of 1,2-cis-diol compounds.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  PERFORMANCE LIQUID-CHROMATOGRAPHY ;  FUNCTIONALIZED MESOPOROUS SILICA ;  POTENTIAL TUMOR-MARKERS ;  URINARY NUCLEOSIDES ;  BREAST-CANCER ;  MS ANALYSIS ;  ACID ;  QUANTIFICATION ;  IDENTIFICATION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000327999800058
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119480
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Li, Hua,Shan, Yuanhong,Qiao, Lizhen,et al. Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites[J]. ANALYTICAL CHEMISTRY,2013,85(23):11585-11592.
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