DICP OpenIR
Subject Area物理化学
Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites
Li, Hua; Shan, Yuanhong; Qiao, Lizhen; Dou, Abo; Shi, Xianzhe; Xu, Guowang; Shi XZ(石先哲); Xu GW(许国旺)
Source PublicationANALYTICAL CHEMISTRY
2013-12-03
DOI10.1021/ac402979w
Volume85Issue:23Pages:11585-11592
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1808
Contribution Rank待补充
WOS HeadingsScience & Technology ; Physical Sciences
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; FUNCTIONALIZED MESOPOROUS SILICA ; POTENTIAL TUMOR-MARKERS ; URINARY NUCLEOSIDES ; BREAST-CANCER ; MS ANALYSIS ; ACID ; QUANTIFICATION ; IDENTIFICATION
AbstractRibosylated metabolites, especially modified nucleosides, have been extensively evaluated as cancer-related biomarkers. Boronate adsorbents are considered to be promising materials for extracting them from complex matrices. However, the enrichment of ribosylated metabolites in low abundance is still a challenge due to the limited capacity and selectivity of the existing boronate adsorbents. In this study, a novel type of magnetic nanoparticles named Fe3O4@SiO2@PEI-FPBA was synthesized by grafting polyethyleneimine (PEI) onto the surface of Fe3O4@SiO2 before modification by boronate groups. The high density of the amino groups on the PEI chains supplied a large number of binding sites for boronate groups. Thus, the adsorption capacity (1.34 +/- 0.024 mg/g) of the nanoparticles, which is 6- to 7-fold higher than that of analogous materials, was greatly improved. The unreacted secondary amines and tertiary amines of the PEI enhanced the aqueous solubility of the nanoparticles, which could efficiently reduce nonspecific adsorption. The nanoparticles were able to capture 1,2 cis-diol nucleosides from 1000-fold interferences. Moreover, the flexible chains of PEI were favorable for effective enrichment and quick equilibration (<2 min). Finally, 60 ribose conjugates were enriched from human urine using the nanoparticles. Among them, 43 were identified to be nucleosides and other ribosylated metabolites. Nine low abundance modified nucleosides were detected for the first time. In conclusion, Fe3O4@SiO2@PEI-FPBA is an attractive candidate material for the highly selective enrichment of 1,2-cis-diol compounds.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
URL查看原文
WOS IDWOS:000327999800058
Citation statistics
Cited Times:65[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119480
Collection中国科学院大连化学物理研究所
Corresponding AuthorShi XZ(石先哲); Xu GW(许国旺)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Li, Hua,Shan, Yuanhong,Qiao, Lizhen,et al. Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites[J]. ANALYTICAL CHEMISTRY,2013,85(23):11585-11592.
APA Li, Hua.,Shan, Yuanhong.,Qiao, Lizhen.,Dou, Abo.,Shi, Xianzhe.,...&许国旺.(2013).Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites.ANALYTICAL CHEMISTRY,85(23),11585-11592.
MLA Li, Hua,et al."Facile Synthesis of Boronate-Decorated Polyethyleneimine-Grafted Hybrid Magnetic Nanoparticles for the Highly Selective Enrichment of Modified Nucleosides and Ribosylated Metabolites".ANALYTICAL CHEMISTRY 85.23(2013):11585-11592.
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