DICP OpenIR
Subject Area物理化学
Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry
Zhang, Junjie1; Zhao, Chunxia1; Chang, Yuwei1; Zhao, Yanni1; Li, Qinghua1,2; Lu, Xin1; Xu, Guowang1; Xu GW(许国旺)
KeywordFree Amino Acids Single Quadrupole Mass Spectrometry Tobacco Ultra-high Performance Liquid Chromatography
Source PublicationJOURNAL OF SEPARATION SCIENCE
2013-09-01
DOI10.1002/jssc.201300450
Volume36Issue:17Pages:2868-2877
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1808
Contribution Rank待补充
WOS HeadingsScience & Technology ; Physical Sciences
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPRECOLUMN DERIVATIZATION ; MS/MS ANALYSIS ; MS ; METABOLISM ; DIAGNOSIS ; HPLC ; TOOL
AbstractAmino acids are one of the most important metabolites of organisms. They play an important role in plant growth, development, and product quality. A method based on RP ultra-performance LC with single quadrupole MS and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate precolumn derivatization was developed for the analysis of free amino acids in flue-cured tobacco leaves. Unlike the corresponding UV detection method, this method avoids matrix interference of complicated tobacco components, and the quantitative accuracy and resolution were improved. Twenty free amino acids were detected in flue-cured tobacco leaves. The method showed a good linearity with correlation coefficients of 0.9966-0.9998. The LODs for derivatized amino acids were 0.2-9.7 fmol/L. Good repeatability with an RSD of 2.5-8.6% and satisfactory intra- and interday precisions were obtained. The developed method was used to investigate free amino acids in flue-cured tobacco leaves in China. The effects of aroma type, variety, and growing regions on free amino acids were investigated. The results showed that free amino acids in tobacco were affected by growing regions and varieties.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
URL查看原文
WOS IDWOS:000323891300018
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119517
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Hongyun Honghe Tobacco Grp, Technol Ctr, Kunming, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Junjie,Zhao, Chunxia,Chang, Yuwei,et al. Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(17):2868-2877.
APA Zhang, Junjie.,Zhao, Chunxia.,Chang, Yuwei.,Zhao, Yanni.,Li, Qinghua.,...&许国旺.(2013).Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry.JOURNAL OF SEPARATION SCIENCE,36(17),2868-2877.
MLA Zhang, Junjie,et al."Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry".JOURNAL OF SEPARATION SCIENCE 36.17(2013):2868-2877.
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