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学科主题物理化学
Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry
Zhang, Junjie1; Zhao, Chunxia1; Chang, Yuwei1; Zhao, Yanni1; Li, Qinghua1,2; Lu, Xin1; Xu, Guowang1; Xu GW(许国旺)
关键词Free Amino Acids Single Quadrupole Mass Spectrometry Tobacco Ultra-high Performance Liquid Chromatography
刊名JOURNAL OF SEPARATION SCIENCE
2013-09-01
DOI10.1002/jssc.201300450
36期:17页:2868-2877
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1808
产权排名待补充
WOS标题词Science & Technology ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PRECOLUMN DERIVATIZATION ; MS/MS ANALYSIS ; MS ; METABOLISM ; DIAGNOSIS ; HPLC ; TOOL
英文摘要Amino acids are one of the most important metabolites of organisms. They play an important role in plant growth, development, and product quality. A method based on RP ultra-performance LC with single quadrupole MS and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate precolumn derivatization was developed for the analysis of free amino acids in flue-cured tobacco leaves. Unlike the corresponding UV detection method, this method avoids matrix interference of complicated tobacco components, and the quantitative accuracy and resolution were improved. Twenty free amino acids were detected in flue-cured tobacco leaves. The method showed a good linearity with correlation coefficients of 0.9966-0.9998. The LODs for derivatized amino acids were 0.2-9.7 fmol/L. Good repeatability with an RSD of 2.5-8.6% and satisfactory intra- and interday precisions were obtained. The developed method was used to investigate free amino acids in flue-cured tobacco leaves in China. The effects of aroma type, variety, and growing regions on free amino acids were investigated. The results showed that free amino acids in tobacco were affected by growing regions and varieties.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
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WOS记录号WOS:000323891300018
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119517
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Hongyun Honghe Tobacco Grp, Technol Ctr, Kunming, Peoples R China
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GB/T 7714
Zhang, Junjie,Zhao, Chunxia,Chang, Yuwei,et al. Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(17):2868-2877.
APA Zhang, Junjie.,Zhao, Chunxia.,Chang, Yuwei.,Zhao, Yanni.,Li, Qinghua.,...&许国旺.(2013).Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry.JOURNAL OF SEPARATION SCIENCE,36(17),2868-2877.
MLA Zhang, Junjie,et al."Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry".JOURNAL OF SEPARATION SCIENCE 36.17(2013):2868-2877.
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