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学科主题: 物理化学
题名: Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry
作者: Zhang, Junjie1;  Zhao, Chunxia1;  Chang, Yuwei1;  Zhao, Yanni1;  Li, Qinghua1, 2;  Lu, Xin1;  Xu, Guowang1
通讯作者: 许国旺
关键词: Free amino acids ;  Single quadrupole mass spectrometry ;  Tobacco ;  Ultra-high performance liquid chromatography
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2013-09-01
DOI: 10.1002/jssc.201300450
卷: 36, 期:17, 页:2868-2877
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Chemistry
英文摘要: Amino acids are one of the most important metabolites of organisms. They play an important role in plant growth, development, and product quality. A method based on RP ultra-performance LC with single quadrupole MS and 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate precolumn derivatization was developed for the analysis of free amino acids in flue-cured tobacco leaves. Unlike the corresponding UV detection method, this method avoids matrix interference of complicated tobacco components, and the quantitative accuracy and resolution were improved. Twenty free amino acids were detected in flue-cured tobacco leaves. The method showed a good linearity with correlation coefficients of 0.9966-0.9998. The LODs for derivatized amino acids were 0.2-9.7 fmol/L. Good repeatability with an RSD of 2.5-8.6% and satisfactory intra- and interday precisions were obtained. The developed method was used to investigate free amino acids in flue-cured tobacco leaves in China. The effects of aroma type, variety, and growing regions on free amino acids were investigated. The results showed that free amino acids in tobacco were affected by growing regions and varieties.
关键词[WOS]: PRECOLUMN DERIVATIZATION ;  MS/MS ANALYSIS ;  MS ;  METABOLISM ;  DIAGNOSIS ;  HPLC ;  TOOL
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000323891300018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119517
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Hongyun Honghe Tobacco Grp, Technol Ctr, Kunming, Peoples R China

Recommended Citation:
Zhang, Junjie,Zhao, Chunxia,Chang, Yuwei,et al. Analysis of free amino acids in flue-cured tobacco leaves using ultra-high performance liquid chromatography with single quadrupole mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(17):2868-2877.
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