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学科主题物理化学
Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase
Long, Zhen; Guo, Zhimou; Xue, Xingya; Zhang, Xiuli; Nordahl, Lilly; Liang, Xinmiao; Zhang XL(张秀莉); Liang XM(梁鑫淼)
关键词Strong Cation Exchange Highly Hydrophilic Bases Purification Natural Product Symmetrical Peak Shape
刊名ANALYTICA CHIMICA ACTA
2013-12-04
DOI10.1016/j.aca.2013.10.034
804页:304-312
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1803
产权排名待补充
WOS标题词Science & Technology ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; REVERSED-PHASE ; RETENTION BEHAVIOR ; PEAK SHAPE ; DRUGS ; HPLC ; IDENTIFICATION ; ENRICHMENT ; ADDITIVES ; SEROTONIN
英文摘要Compared to moderately and weakly hydrophilic bases, highly polar basic compounds are even more difficult to separate due to their poor retention in reversed phase (RP) mode. This study described the successful applications of a strong cation exchange (SCX) stationary phase to achieve symmetric peak shape, adequate retention and selectivity in the separation of very polar basic compounds. Salt and acetonitrile concentrations were adjusted to optimize the separation. Good correlations (R-2 = 0.998-1.000) between the logarithm of the retention factor and the logarithm of salt or acetonitrile concentration were obtained. Gradients generated by changing salt or acetonitrile concentration were compared for the analysis of different highly polar bases. Although all of the analytes were eluted more quickly with an acetonitrile gradient, the effect of the gradients tested on peak width and peak shape varied with respect to analyte. In addition, the effects of different types of cation and anion additives were also investigated. After separation parameters were acquired, the SCX-based method was utilized to analyze highly hydrophilic alkaloids from Scopolia tangutica Maxim with high separation efficiency (plate numbers >32,000 m(-1)). Concurrently, one very polar alkaloid fraction was purified with symmetric peak shape using the current method. Our results suggest that SCX stationary phase can be used as an alternative to RP stationary phase in the analysis and purification of highly hydrophilic basic compounds. (C) 2013 Elsevier B.V. All rights reserved.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
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WOS记录号WOS:000327493500040
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119531
专题中国科学院大连化学物理研究所
通讯作者Zhang XL(张秀莉); Liang XM(梁鑫淼)
作者单位Dalian Univ Technol, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Long, Zhen,Guo, Zhimou,Xue, Xingya,et al. Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase[J]. ANALYTICA CHIMICA ACTA,2013,804:304-312.
APA Long, Zhen.,Guo, Zhimou.,Xue, Xingya.,Zhang, Xiuli.,Nordahl, Lilly.,...&梁鑫淼.(2013).Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase.ANALYTICA CHIMICA ACTA,804,304-312.
MLA Long, Zhen,et al."Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase".ANALYTICA CHIMICA ACTA 804(2013):304-312.
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