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学科主题: 物理化学
题名: Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase
作者: Long, Zhen1;  Guo, Zhimou1;  Xue, Xingya1;  Zhang, Xiuli1;  Nordahl, Lilly1;  Liang, Xinmiao1
通讯作者: 张秀莉 ;  梁鑫淼
关键词: Strong cation exchange ;  Highly hydrophilic bases ;  Purification ;  Natural product ;  Symmetrical peak shape
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2013-12-04
DOI: 10.1016/j.aca.2013.10.034
卷: 804, 页:304-312
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1803
产权排名: 待补充
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Chemistry
英文摘要: Compared to moderately and weakly hydrophilic bases, highly polar basic compounds are even more difficult to separate due to their poor retention in reversed phase (RP) mode. This study described the successful applications of a strong cation exchange (SCX) stationary phase to achieve symmetric peak shape, adequate retention and selectivity in the separation of very polar basic compounds. Salt and acetonitrile concentrations were adjusted to optimize the separation. Good correlations (R-2 = 0.998-1.000) between the logarithm of the retention factor and the logarithm of salt or acetonitrile concentration were obtained. Gradients generated by changing salt or acetonitrile concentration were compared for the analysis of different highly polar bases. Although all of the analytes were eluted more quickly with an acetonitrile gradient, the effect of the gradients tested on peak width and peak shape varied with respect to analyte. In addition, the effects of different types of cation and anion additives were also investigated. After separation parameters were acquired, the SCX-based method was utilized to analyze highly hydrophilic alkaloids from Scopolia tangutica Maxim with high separation efficiency (plate numbers >32,000 m(-1)). Concurrently, one very polar alkaloid fraction was purified with symmetric peak shape using the current method. Our results suggest that SCX stationary phase can be used as an alternative to RP stationary phase in the analysis and purification of highly hydrophilic basic compounds. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  REVERSED-PHASE ;  RETENTION BEHAVIOR ;  PEAK SHAPE ;  DRUGS ;  HPLC ;  IDENTIFICATION ;  ENRICHMENT ;  ADDITIVES ;  SEROTONIN
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000327493500040
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119531
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Long, Zhen,Guo, Zhimou,Xue, Xingya,et al. Selective separation and purification of highly polar basic compounds using a silica-based strong cation exchange stationary phase[J]. ANALYTICA CHIMICA ACTA,2013,804:304-312.
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