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学科主题物理化学
A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis
Wang, Shuangyuan; Li, Jia; Shi, Xianzhe; Qiao, Lizhen; Lu, Xin; Xu, Guowang; Shi XZ(石先哲); Xu GW(许国旺)
关键词Stop-flow On-line Two-dimensional Liquid Chromatography Lipid Ms
刊名JOURNAL OF CHROMATOGRAPHY A
2013-12-20
DOI10.1016/j.chroma.2013.10.069
1321页:65-72
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1808
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HYDROPHILIC INTERACTION ; PHOSPHOLIPID CLASSES ; BIOLOGICAL SAMPLES ; CRUDE EXTRACTS ; HUMAN BLOOD ; SEPARATION ; PLASMA ; IDENTIFICATION ; TEMPERATURE ; RPLC
英文摘要A novel on-line two dimensional liquid chromatography (2D LC) based on stop-flow mode coupled with electrospray ionization mass spectrometry (ESI-MS) method was established to separate lipids in human plasma. Hydrophilic interaction liquid chromatography (HILIC) in the first dimension and reversed-phase liquid chromatography (RP LC) in the second dimension were used to separate the lipids into six fractions based on their polar head groups and further into peaks based on aliphatic chains, respectively. A new stop-flow interface with a trap column and an extra make-up flow was designed to construct this system and trap the components eluted from the first dimension. Moreover, the same length of analytical columns and similar flow rates were used in the first and second dimensions. Therefore, the new stop-flow 2D LC system can avoid the sensitivity decrease caused by the dilution effect, which is the shortcoming of comprehensive 2D LC. Three hundred and seventy-two lipids were identified from plasma extract using this 2D LC coupled with ESI-MS in positive mode, and 88 more lipids were detected than one-dimensional RP LC analysis. Peak capacity of this stop-flow 2D LC was 415, which is similar to that of comprehensive 2D LC. The linearity, repeatability and sensitivity of this method were satisfactory, which demonstrated that this method was also suitable for quantitative analysis. All these results indicated that this on-line 2D LC method is powerful for qualitative and quantitative analysis of complex lipids (C) 2013 Elsevier B.V. All rights reserved.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
原文出处查看原文
WOS记录号WOS:000328596400008
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119533
专题中国科学院大连化学物理研究所
通讯作者Shi XZ(石先哲); Xu GW(许国旺)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Wang, Shuangyuan,Li, Jia,Shi, Xianzhe,et al. A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1321:65-72.
APA Wang, Shuangyuan.,Li, Jia.,Shi, Xianzhe.,Qiao, Lizhen.,Lu, Xin.,...&许国旺.(2013).A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis.JOURNAL OF CHROMATOGRAPHY A,1321,65-72.
MLA Wang, Shuangyuan,et al."A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis".JOURNAL OF CHROMATOGRAPHY A 1321(2013):65-72.
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