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学科主题: 物理化学
题名: A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis
作者: Wang, Shuangyuan1;  Li, Jia1;  Shi, Xianzhe1;  Qiao, Lizhen1;  Lu, Xin1;  Xu, Guowang1
通讯作者: 石先哲 ;  许国旺
关键词: Stop-flow ;  On-line ;  Two-dimensional liquid chromatography ;  Lipid ;  MS
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2013-12-20
DOI: 10.1016/j.chroma.2013.10.069
卷: 1321, 页:65-72
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1808
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A novel on-line two dimensional liquid chromatography (2D LC) based on stop-flow mode coupled with electrospray ionization mass spectrometry (ESI-MS) method was established to separate lipids in human plasma. Hydrophilic interaction liquid chromatography (HILIC) in the first dimension and reversed-phase liquid chromatography (RP LC) in the second dimension were used to separate the lipids into six fractions based on their polar head groups and further into peaks based on aliphatic chains, respectively. A new stop-flow interface with a trap column and an extra make-up flow was designed to construct this system and trap the components eluted from the first dimension. Moreover, the same length of analytical columns and similar flow rates were used in the first and second dimensions. Therefore, the new stop-flow 2D LC system can avoid the sensitivity decrease caused by the dilution effect, which is the shortcoming of comprehensive 2D LC. Three hundred and seventy-two lipids were identified from plasma extract using this 2D LC coupled with ESI-MS in positive mode, and 88 more lipids were detected than one-dimensional RP LC analysis. Peak capacity of this stop-flow 2D LC was 415, which is similar to that of comprehensive 2D LC. The linearity, repeatability and sensitivity of this method were satisfactory, which demonstrated that this method was also suitable for quantitative analysis. All these results indicated that this on-line 2D LC method is powerful for qualitative and quantitative analysis of complex lipids (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: HYDROPHILIC INTERACTION ;  PHOSPHOLIPID CLASSES ;  BIOLOGICAL SAMPLES ;  CRUDE EXTRACTS ;  HUMAN BLOOD ;  SEPARATION ;  PLASMA ;  IDENTIFICATION ;  TEMPERATURE ;  RPLC
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000328596400008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119533
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Shuangyuan,Li, Jia,Shi, Xianzhe,et al. A novel stop-flow two-dimensional liquid chromatography-mass spectrometry method for lipid analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1321:65-72.
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