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学科主题物理化学
Mass Defect-Based Pseudo-Isobaric Dimethyl Labeling for Proteome Quantification
Zhou, Yuan1,2; Shan, Yichu1; Wu, Qi1,2; Zhang, Shen1,2; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
刊名ANALYTICAL CHEMISTRY
2013-11-19
DOI10.1021/ac402834w
85期:22页:10658-10663
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1810
产权排名待补充
WOS标题词Science & Technology ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]QUANTITATIVE PROTEOMICS ; PEPTIDE ; EXPRESSION ; ACCURATE ; IDENTIFICATION ; SPECTROMETRY
英文摘要Discovering differentially expressed proteins in various biological samples requires proteome quantification methods with accuracy, precision, and wide dynamic range. This study describes a mass defect-based pseudo-isobaric dimethyl labeling (pIDL) method based on the subtle mass defect differences between C-12/C-13 and H-1/H-2. Lys-C protein digests were labeled with CD2O/(CD2O)-C-13 and reduced with NaCNBD3/NaCNBH3 as heavy and light isotopologues, respectively. The fragment ion pairs with mass differences of 5.84 mDa were resolved by high-resolution tandem mass spectrometry (MS/MS) and used for quantification. The pIDL method described here resulted in highly accurate and precise quantification results with approximately 100-fold dynamic range. Furthermore, the pIDL method was extended to 4-plex proteome quantification and applied to the quantitative analysis of proteomes from Hca-P and Hca-F, two mouse hepatocarcinoma ascites syngeneic cell lines with low and high lymph node metastasis rates.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
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WOS记录号WOS:000327360900006
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119543
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
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GB/T 7714
Zhou, Yuan,Shan, Yichu,Wu, Qi,et al. Mass Defect-Based Pseudo-Isobaric Dimethyl Labeling for Proteome Quantification[J]. ANALYTICAL CHEMISTRY,2013,85(22):10658-10663.
APA Zhou, Yuan.,Shan, Yichu.,Wu, Qi.,Zhang, Shen.,Zhang, Lihua.,...&张丽华.(2013).Mass Defect-Based Pseudo-Isobaric Dimethyl Labeling for Proteome Quantification.ANALYTICAL CHEMISTRY,85(22),10658-10663.
MLA Zhou, Yuan,et al."Mass Defect-Based Pseudo-Isobaric Dimethyl Labeling for Proteome Quantification".ANALYTICAL CHEMISTRY 85.22(2013):10658-10663.
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