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学科主题物理化学
Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow
Bian, Yangyang1,2; Ye, Mingliang1; Wang, Chunli1,2; Cheng, Kai1,2; Song, Chunxia1,2; Dong, Mingming1,2; Pan, Yanbo1,2; Qin, Hongqiang1,2; Zou, Hanfa1; Ye ML(叶明亮); Zou HF(邹汉法)
刊名SCIENTIFIC REPORTS
2013-12-10
ISSN2045-2322
DOI10.1038/srep03460
3页:03460
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1809
产权排名待补充
WOS标题词Science & Technology
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]PROTEIN-PHOSPHORYLATION ; COMPREHENSIVE RESOURCE ; IDENTIFICATION ; SPLICEOSOME ; COMPLEXES ; REVEALS ; ATLAS ; MOUSE ; ASSAY
英文摘要Due to its constitutive activity and ubiquitous distribution, CK2 is the most pleiotropic kinase among the individual members of the protein kinase superfamily. Identification of CK2 substrates is vital to decipher its role in biological processes. However, only a limited number of CK2 substrates were identified so far. In this study, we developed an integrated phosphoproteomics workflow to identify the CK2 substrates in large scale. First, in vitro kinase reactions with immobilized proteomes were combined with quantitative phosphoproteomics to identify in vitro CK2 phosphorylation sites, which leaded to identification of 988 sites from 581 protein substrates. To reduce false positives, we proposed an approach by comparing these in vitro sites with the public databases that collect in vivo phosphorylation sites. After the removal of the sites that were excluded in the databases, 605 high confident CK2 sites corresponding to 356 proteins were retained. The CK2 substrates identified in this study were based on the discovery mode, in which an unbiased overview of CK2 substrates was provided. Our result revealed that CK2 substrates were significantly enriched in the spliceosomal proteins, indicating CK2 might regulate the functions of spliceosome.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
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WOS记录号WOS:000328125600003
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119581
专题中国科学院大连化学物理研究所
通讯作者Ye ML(叶明亮); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Bian, Yangyang,Ye, Mingliang,Wang, Chunli,et al. Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow[J]. SCIENTIFIC REPORTS,2013,3:03460.
APA Bian, Yangyang.,Ye, Mingliang.,Wang, Chunli.,Cheng, Kai.,Song, Chunxia.,...&邹汉法.(2013).Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow.SCIENTIFIC REPORTS,3,03460.
MLA Bian, Yangyang,et al."Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow".SCIENTIFIC REPORTS 3(2013):03460.
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