中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow
作者: Bian, Yangyang1, 2;  Ye, Mingliang1;  Wang, Chunli1, 2;  Cheng, Kai1, 2;  Song, Chunxia1, 2;  Dong, Mingming1, 2;  Pan, Yanbo1, 2;  Qin, Hongqiang1, 2;  Zou, Hanfa1
通讯作者: 叶明亮 ;  邹汉法
刊名: SCIENTIFIC REPORTS
发表日期: 2013-12-10
DOI: 10.1038/srep03460
卷: 3, 页:03460
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
资助者: 1,1
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Due to its constitutive activity and ubiquitous distribution, CK2 is the most pleiotropic kinase among the individual members of the protein kinase superfamily. Identification of CK2 substrates is vital to decipher its role in biological processes. However, only a limited number of CK2 substrates were identified so far. In this study, we developed an integrated phosphoproteomics workflow to identify the CK2 substrates in large scale. First, in vitro kinase reactions with immobilized proteomes were combined with quantitative phosphoproteomics to identify in vitro CK2 phosphorylation sites, which leaded to identification of 988 sites from 581 protein substrates. To reduce false positives, we proposed an approach by comparing these in vitro sites with the public databases that collect in vivo phosphorylation sites. After the removal of the sites that were excluded in the databases, 605 high confident CK2 sites corresponding to 356 proteins were retained. The CK2 substrates identified in this study were based on the discovery mode, in which an unbiased overview of CK2 substrates was provided. Our result revealed that CK2 substrates were significantly enriched in the spliceosomal proteins, indicating CK2 might regulate the functions of spliceosome.
关键词[WOS]: PROTEIN-PHOSPHORYLATION ;  COMPREHENSIVE RESOURCE ;  IDENTIFICATION ;  SPLICEOSOME ;  COMPLEXES ;  REVEALS ;  ATLAS ;  MOUSE ;  ASSAY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000328125600003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119581
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2013uSKktXWWQx.PDF(687KB)----开放获取View Download

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Bian, Yangyang,Ye, Mingliang,Wang, Chunli,et al. Global Screening of CK2 Kinase Substrates by an Integrated Phosphoproteomics Workflow[J]. SCIENTIFIC REPORTS,2013,3:03460.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Bian, Yangyang]'s Articles
 [Ye, Mingliang]'s Articles
 [Wang, Chunli]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Bian, Yangyang]‘s Articles
 [Ye, Mingliang]‘s Articles
 [Wang, Chunli]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2013uSKktXWWQx.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace