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学科主题: 物理化学
题名: An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus
作者: Zhong, Weiliang1;  Ma, Huipeng2;  Wang, Shouyu1;  Gao, Xinghua2;  Zhang, Weiguo1;  Qin, Jianhua2
通讯作者: Weiguo Zhang
关键词: Microfluidic ;  Flow shear stress ;  Chondrocyte ;  Mechanical microenvironment
刊名: MICROFLUIDICS AND NANOFLUIDICS
发表日期: 2013-12-01
DOI: 10.1007/s10404-013-1186-9
卷: 15, 期:6, 页:763-773
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1807
产权排名: 待补充
WOS标题词: Science & Technology ;  Technology ;  Physical Sciences
类目[WOS]: Nanoscience & Nanotechnology ;  Instruments & Instrumentation ;  Physics, Fluids & Plasmas
资助者: 2,2
研究领域[WOS]: Science & Technology - Other Topics ;  Instruments & Instrumentation ;  Physics
英文摘要: In this work, we presented a novel integrated microfluidic perfusion system to generate multiple parameter fluid flow-induced shear stresses simultaneously and investigated the effects of distinct levels of fluid flow stimulus on the responses of chondrocytes, including the changes of morphology and metabolism. Based on the electric circuit analogy, two devices were fabricated, each with four chambers to enable eight different shear stresses spanning over four orders of magnitude from 0.007 to 15.4 dyne/cm(2) with computational fluid dynamics analysis. Chondrocytes subjected to shear stresses (7.5 and 15.4 dyne/cm(2)) for 24 h reoriented their cytoskeleton to align with the direction of flow. Meanwhile, the collagen I, collagen II and aggrecan expression of chondrocytes increased in different ranges, respectively. Furthermore, interleukin-6 as a proinflammatory cytokine can be detected at shear stress of 7.5 and 15.4 dyne/cm(2) in mRNA level. These results indicated that fluid flow was beneficial for chondrocyte metabolism at interstitial levels (0.007 and 0.046 dyne/cm(2)), but induced an increase in fibrocartilage phenotype with increasing magnitude of stimulation. Moreover, a moderate level of flow stimulus (7.5 dyne/cm(2)) could also result in detrimental cytokine release. This work described a simple and versatile way to rapidly screen cell responses to fluid flow stimulus from interstitial shear stress level to pathological level, providing multi-condition fluid flow-induced microenvironment in vitro for understanding deeply chondrocyte metabolism, cartilage reconstruction and osteoarthritis etiology.
关键词[WOS]: HUMAN OSTEOARTHRITIC CHONDROCYTES ;  TENSILE MECHANICAL-PROPERTIES ;  SHEAR-STRESS ;  ARTICULAR-CARTILAGE ;  INTERSTITIAL FLOW ;  NITRIC-OXIDE ;  IN-VITRO ;  TISSUE ;  CELLS ;  EXPRESSION
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000327131200004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119635
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Dept Orthopaed, Affiliated Hosp 1, Dalian, Peoples R China
2.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Zhong, Weiliang,Ma, Huipeng,Wang, Shouyu,et al. An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus[J]. MICROFLUIDICS AND NANOFLUIDICS,2013,15(6):763-773.
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