DICP OpenIR
Subject Area物理化学
An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus
Zhong, Weiliang1; Ma, Huipeng2; Wang, Shouyu1; Gao, Xinghua2; Zhang, Weiguo1; Qin, Jianhua2; Weiguo Zhang
KeywordMicrofluidic Flow Shear Stress Chondrocyte Mechanical Microenvironment
Source PublicationMICROFLUIDICS AND NANOFLUIDICS
2013-12-01
ISSN1613-4982
DOI10.1007/s10404-013-1186-9
Volume15Issue:6Pages:763-773
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1807
Contribution Rank待补充
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
Funding Organization2,2 ; 2,2 ; 2,2 ; 2,2
WOS SubjectNanoscience & Nanotechnology ; Instruments & Instrumentation ; Physics, Fluids & Plasmas
WOS Research AreaScience & Technology - Other Topics ; Instruments & Instrumentation ; Physics
WOS KeywordHUMAN OSTEOARTHRITIC CHONDROCYTES ; TENSILE MECHANICAL-PROPERTIES ; SHEAR-STRESS ; ARTICULAR-CARTILAGE ; INTERSTITIAL FLOW ; NITRIC-OXIDE ; IN-VITRO ; TISSUE ; CELLS ; EXPRESSION
AbstractIn this work, we presented a novel integrated microfluidic perfusion system to generate multiple parameter fluid flow-induced shear stresses simultaneously and investigated the effects of distinct levels of fluid flow stimulus on the responses of chondrocytes, including the changes of morphology and metabolism. Based on the electric circuit analogy, two devices were fabricated, each with four chambers to enable eight different shear stresses spanning over four orders of magnitude from 0.007 to 15.4 dyne/cm(2) with computational fluid dynamics analysis. Chondrocytes subjected to shear stresses (7.5 and 15.4 dyne/cm(2)) for 24 h reoriented their cytoskeleton to align with the direction of flow. Meanwhile, the collagen I, collagen II and aggrecan expression of chondrocytes increased in different ranges, respectively. Furthermore, interleukin-6 as a proinflammatory cytokine can be detected at shear stress of 7.5 and 15.4 dyne/cm(2) in mRNA level. These results indicated that fluid flow was beneficial for chondrocyte metabolism at interstitial levels (0.007 and 0.046 dyne/cm(2)), but induced an increase in fibrocartilage phenotype with increasing magnitude of stimulation. Moreover, a moderate level of flow stimulus (7.5 dyne/cm(2)) could also result in detrimental cytokine release. This work described a simple and versatile way to rapidly screen cell responses to fluid flow stimulus from interstitial shear stress level to pathological level, providing multi-condition fluid flow-induced microenvironment in vitro for understanding deeply chondrocyte metabolism, cartilage reconstruction and osteoarthritis etiology.
Language英语
Funding Organization2,2 ; 2,2 ; 2,2 ; 2,2
URL查看原文
WOS IDWOS:000327131200004
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119635
Collection中国科学院大连化学物理研究所
Corresponding AuthorWeiguo Zhang
Affiliation1.Dalian Med Univ, Dept Orthopaed, Affiliated Hosp 1, Dalian, Peoples R China
2.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Zhong, Weiliang,Ma, Huipeng,Wang, Shouyu,et al. An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus[J]. MICROFLUIDICS AND NANOFLUIDICS,2013,15(6):763-773.
APA Zhong, Weiliang.,Ma, Huipeng.,Wang, Shouyu.,Gao, Xinghua.,Zhang, Weiguo.,...&Weiguo Zhang.(2013).An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus.MICROFLUIDICS AND NANOFLUIDICS,15(6),763-773.
MLA Zhong, Weiliang,et al."An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus".MICROFLUIDICS AND NANOFLUIDICS 15.6(2013):763-773.
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