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学科主题物理化学
Large-scaled human serum sphingolipid profiling by using reversed-phase liquid chromatography coupled with dynamic multiple reaction monitoring of mass spectrometry: Method development and application in hepatocellular carcinoma
Li, Jia; Hu, Chunxiu; Zhao, Xinjie; Dai, Weidong; Chen, Shili; Lu, Xin; Xu, Guowang; Xu GW(许国旺)
关键词Sphingolipid Profiling Lipidomics Liquid Chromatography-mass Spectrometry Multiple Reaction Monitoring Tert-butyl Methyl Ether Serum
刊名JOURNAL OF CHROMATOGRAPHY A
2013-12-13
DOI10.1016/j.chroma.2013.10.064
1320页:103-110
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1808
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]SIMULTANEOUS QUANTITATIVE-ANALYSIS ; TERT-BUTYL ETHER ; CELLULAR-EXTRACTS ; LIPID EXTRACTION ; HIGH-THROUGHPUT ; HUMAN PLASMA ; CERAMIDE ; METABOLISM ; CANCER ; QUANTIFICATION
英文摘要Sphingolipids are a family of bioactive molecules with high structural diversity and complexity. They not only serve as integral components of cellular membrane, but also play pivotal roles in signaling and other cellular events. It is desirable for the development of sensitive, robust and structural-specific analytical approaches enabling rapid determination of as many sphingolipid species as possible. Herein we present an analytical method for large-scaled profiling of sphigolipids in human serum, which consisted of an improved extraction protocol using tert-butyl methyl ether combined with mild alkaline hydrolysis, and an ultra high performance reversed-phase liquid chromatography-dynamic multiple reaction monitoring-mass spectrometric (RPLC-dynamic MRM-MS) method. In total 84 endogenous sphingolipid species covering six subcategories (i.e. free sphingoid base, dihydroceramide, ceramide, hexosylceramide, lactosylceramide, and sphingomyelin), were separated and quantified in a single run within 10 min. A broad linear range over 2.5-4 orders of magnitude (r(2)>0.99), a limit of detection of 0.01-0.17 pmol/mL, and a limit of quantitation of 0.02-0.42 pmol/mL were obtained for each subcategory. Average recovery of each subcategory was within 85.6-95.6%. Median values of coefficient of variation (CV) of all detected 84 sphingolipids were 3.9% and 6.8% for intraday and interday precision, respectively. This method was exemplarily applied in a study regarding dysregulated sphingolipid homeostasis in hepatocellular carcinoma. The establishment of this method provides a useful tool for serum-based high throughput screening of sphingolipid biomarkers and mechanism investigation of sphingolipid metabolic regulation in human disease. (C) 2013 Elsevier B.V. All rights reserved.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
原文出处查看原文
WOS记录号WOS:000327922100012
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119653
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Li, Jia,Hu, Chunxiu,Zhao, Xinjie,et al. Large-scaled human serum sphingolipid profiling by using reversed-phase liquid chromatography coupled with dynamic multiple reaction monitoring of mass spectrometry: Method development and application in hepatocellular carcinoma[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1320:103-110.
APA Li, Jia.,Hu, Chunxiu.,Zhao, Xinjie.,Dai, Weidong.,Chen, Shili.,...&许国旺.(2013).Large-scaled human serum sphingolipid profiling by using reversed-phase liquid chromatography coupled with dynamic multiple reaction monitoring of mass spectrometry: Method development and application in hepatocellular carcinoma.JOURNAL OF CHROMATOGRAPHY A,1320,103-110.
MLA Li, Jia,et al."Large-scaled human serum sphingolipid profiling by using reversed-phase liquid chromatography coupled with dynamic multiple reaction monitoring of mass spectrometry: Method development and application in hepatocellular carcinoma".JOURNAL OF CHROMATOGRAPHY A 1320(2013):103-110.
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