DICP OpenIR
Subject Area物理化学
Large-scaled human serum sphingolipid profiling by using reversed-phase liquid chromatography coupled with dynamic multiple reaction monitoring of mass spectrometry: Method development and application in hepatocellular carcinoma
Li, Jia; Hu, Chunxiu; Zhao, Xinjie; Dai, Weidong; Chen, Shili; Lu, Xin; Xu, Guowang; Xu GW(许国旺)
KeywordSphingolipid Profiling Lipidomics Liquid Chromatography-mass Spectrometry Multiple Reaction Monitoring Tert-butyl Methyl Ether Serum
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2013-12-13
DOI10.1016/j.chroma.2013.10.064
Volume1320Pages:103-110
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1808
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordSIMULTANEOUS QUANTITATIVE-ANALYSIS ; TERT-BUTYL ETHER ; CELLULAR-EXTRACTS ; LIPID EXTRACTION ; HIGH-THROUGHPUT ; HUMAN PLASMA ; CERAMIDE ; METABOLISM ; CANCER ; QUANTIFICATION
AbstractSphingolipids are a family of bioactive molecules with high structural diversity and complexity. They not only serve as integral components of cellular membrane, but also play pivotal roles in signaling and other cellular events. It is desirable for the development of sensitive, robust and structural-specific analytical approaches enabling rapid determination of as many sphingolipid species as possible. Herein we present an analytical method for large-scaled profiling of sphigolipids in human serum, which consisted of an improved extraction protocol using tert-butyl methyl ether combined with mild alkaline hydrolysis, and an ultra high performance reversed-phase liquid chromatography-dynamic multiple reaction monitoring-mass spectrometric (RPLC-dynamic MRM-MS) method. In total 84 endogenous sphingolipid species covering six subcategories (i.e. free sphingoid base, dihydroceramide, ceramide, hexosylceramide, lactosylceramide, and sphingomyelin), were separated and quantified in a single run within 10 min. A broad linear range over 2.5-4 orders of magnitude (r(2)>0.99), a limit of detection of 0.01-0.17 pmol/mL, and a limit of quantitation of 0.02-0.42 pmol/mL were obtained for each subcategory. Average recovery of each subcategory was within 85.6-95.6%. Median values of coefficient of variation (CV) of all detected 84 sphingolipids were 3.9% and 6.8% for intraday and interday precision, respectively. This method was exemplarily applied in a study regarding dysregulated sphingolipid homeostasis in hepatocellular carcinoma. The establishment of this method provides a useful tool for serum-based high throughput screening of sphingolipid biomarkers and mechanism investigation of sphingolipid metabolic regulation in human disease. (C) 2013 Elsevier B.V. All rights reserved.
Language英语
Funding Organization1,1 ; 1,1 ; 1,1 ; 1,1
URL查看原文
WOS IDWOS:000327922100012
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119653
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Li, Jia,Hu, Chunxiu,Zhao, Xinjie,et al. Large-scaled human serum sphingolipid profiling by using reversed-phase liquid chromatography coupled with dynamic multiple reaction monitoring of mass spectrometry: Method development and application in hepatocellular carcinoma[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1320:103-110.
APA Li, Jia.,Hu, Chunxiu.,Zhao, Xinjie.,Dai, Weidong.,Chen, Shili.,...&许国旺.(2013).Large-scaled human serum sphingolipid profiling by using reversed-phase liquid chromatography coupled with dynamic multiple reaction monitoring of mass spectrometry: Method development and application in hepatocellular carcinoma.JOURNAL OF CHROMATOGRAPHY A,1320,103-110.
MLA Li, Jia,et al."Large-scaled human serum sphingolipid profiling by using reversed-phase liquid chromatography coupled with dynamic multiple reaction monitoring of mass spectrometry: Method development and application in hepatocellular carcinoma".JOURNAL OF CHROMATOGRAPHY A 1320(2013):103-110.
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