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学科主题: 物理化学
题名: An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots
作者: Sui, Tao1;  Ge, Yingbin2;  Liu, Wujun3;  Zhao, Zongbao K.3;  Zhang, Ning1;  Cao, Xiaojian1
通讯作者: 赵宗保 ;  Zhang N ;  Cao XJ
刊名: PLOS ONE
发表日期: 2013-07-23
DOI: 10.1371/journal.pone.0069049
卷: 8, 期:7, 页:69049
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1816
产权排名: 待补充
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
资助者: 3,3
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Differences in the levels of acetylcholinesterase (AChE) in ventral and dorsal spinal roots can be used to differentiate the spinal nerves. Although many methods are available to assay AChE, a rapid and sensitive method has not been previously developed. Here, we describe an antibody-based quartz crystal microbalance (QCM) assay and its application for the quantification of AChE in the solutions of ventral and dorsal spinal roots. The frequency variation of the QCM device corresponds to the level of AChE over a wide dynamic range (0.5-10 mu g/ml), which is comparable to the response range of the ELISA method. The frequency shift caused by the ventral roots is 3-fold greater than that caused by the dorsal roots. The antibody-based QCM sensor was stable across many successive replicate samples, and the method required less than 10 min, including the AChE extraction and analysis steps. This method is a rapid and convenient means for the quantification of AChE in biological samples and may be applicable for distinguishing the ventral and dorsal roots during surgical operations.
关键词[WOS]: PERIPHERAL-NERVE ;  HISTOCHEMICAL IDENTIFICATION ;  IMMOBILIZATION METHODS ;  SENSORY FASCICLES ;  MOTOR ;  DISSIPATION ;  NEURONS ;  IMMUNOHISTOCHEMISTRY ;  NANOPARTICLES ;  IMMUNOSENSOR
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000325211000071
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119668
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nanjing Med Univ, Affiliated Hosp 1, Dept Orthoped, Nanjing, Jiangsu, Peoples R China
2.Nanjing Med Univ, Dept Physiol, Nanjing, Jiangsu, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian, Peoples R China

Recommended Citation:
Sui, Tao,Ge, Yingbin,Liu, Wujun,et al. An Acetylcholinesterase Antibody-Based Quartz Crystal Microbalance for the Rapid Identification of Spinal Ventral and Dorsal Roots[J]. PLOS ONE,2013,8(7):69049.
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