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学科主题物理化学
Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood
Cao, Jiali1; Zhang, Xihao1; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,2; Chen LX(陈朗星); Zhang YK(张玉奎)
刊名JOURNAL OF MATERIALS CHEMISTRY B
2013
DOI10.1039/c3tb20573h
1期:30页:3625-3632
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1810
产权排名待补充
WOS标题词Science & Technology ; Technology
资助者2,5 ; 2,5 ; 2,5 ; 2,5
类目[WOS]Materials Science, Biomaterials
研究领域[WOS]Materials Science
关键词[WOS]HISTIDINE-TAGGED PROTEINS ; CORE/SHELL NANOPARTICLES ; BIOMEDICAL APPLICATIONS ; MICROSPHERES ; CHROMATOGRAPHY ; EXTRACTION ; SEPARATION ; FE3O4/AU ; SURFACE ; SAMPLES
英文摘要In this study, a facile and efficient separation of abundant proteins from bovine blood using core-shell structure nanoparticles with a magnetic core and an immobilized metal affinity ligand iminodiacetic acid (IDA) chelating Ni(II) is presented. Firstly, Fe3O4 magnetic nanoparticles (MNPs) were synthesized through a solvothermal method and then were conveniently surface-modified with 3-(methacryloyloxy) propyltrimethoxylsilane as anchor molecules to donate vinyl groups. Next a high density poly(4-vinylbenzylchloride) (PVBC) shell was synthesized on the surface of silica-coated Fe3O4 MNPs via distillation-precipitation polymerization. After the PVBC shell reacted with iminodiacetic acid (IDA) in alkaline aqueous solution, the magnetite was charged with Ni2+ to form Ni(II)-IDA functionalized hybrid Fe3O4@PVBC@IDA-Ni MNPs. Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA) and a vibrating sample magnetometer (VSM) were employed to evaluate the size, morphology and magnetic property of the resulting magnetic nanospheres. The high saturation magnetization (48.1 emu g(-1)) provides the materials with the convenience of magnetic separation under an external magnetic field and they can be subsequently reused. The core-shell Fe3O4@PVBC@IDA-Ni MNPs exhibit excellent performance in the separation of protein bovine hemoglobin (BHb), and the binding capacity is as high as 1988 mg g(-1). In addition, the Fe3O4@PVBC@IDA-Ni MNPs can be used in selective removal of abundant protein Hb in the bovine blood samples. This opens a novel route for its future application in removing abundant protein in proteomic analysis.
语种英语
资助者2,5 ; 2,5 ; 2,5 ; 2,5
原文出处查看原文
WOS记录号WOS:000321626200001
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119674
专题中国科学院大连化学物理研究所
通讯作者Chen LX(陈朗星); Zhang YK(张玉奎)
作者单位1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
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GB/T 7714
Cao, Jiali,Zhang, Xihao,He, Xiwen,et al. Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood[J]. JOURNAL OF MATERIALS CHEMISTRY B,2013,1(30):3625-3632.
APA Cao, Jiali.,Zhang, Xihao.,He, Xiwen.,Chen, Langxing.,Zhang, Yukui.,...&张玉奎.(2013).Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood.JOURNAL OF MATERIALS CHEMISTRY B,1(30),3625-3632.
MLA Cao, Jiali,et al."Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood".JOURNAL OF MATERIALS CHEMISTRY B 1.30(2013):3625-3632.
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