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学科主题: 物理化学
题名: Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood
作者: Cao, Jiali1;  Zhang, Xihao1;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 2
通讯作者: 陈朗星 ;  张玉奎
刊名: JOURNAL OF MATERIALS CHEMISTRY B
发表日期: 2013
DOI: 10.1039/c3tb20573h
卷: 1, 期:30, 页:3625-3632
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 待补充
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Materials Science, Biomaterials
资助者: 2,5
研究领域[WOS]: Materials Science
英文摘要: In this study, a facile and efficient separation of abundant proteins from bovine blood using core-shell structure nanoparticles with a magnetic core and an immobilized metal affinity ligand iminodiacetic acid (IDA) chelating Ni(II) is presented. Firstly, Fe3O4 magnetic nanoparticles (MNPs) were synthesized through a solvothermal method and then were conveniently surface-modified with 3-(methacryloyloxy) propyltrimethoxylsilane as anchor molecules to donate vinyl groups. Next a high density poly(4-vinylbenzylchloride) (PVBC) shell was synthesized on the surface of silica-coated Fe3O4 MNPs via distillation-precipitation polymerization. After the PVBC shell reacted with iminodiacetic acid (IDA) in alkaline aqueous solution, the magnetite was charged with Ni2+ to form Ni(II)-IDA functionalized hybrid Fe3O4@PVBC@IDA-Ni MNPs. Transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA) and a vibrating sample magnetometer (VSM) were employed to evaluate the size, morphology and magnetic property of the resulting magnetic nanospheres. The high saturation magnetization (48.1 emu g(-1)) provides the materials with the convenience of magnetic separation under an external magnetic field and they can be subsequently reused. The core-shell Fe3O4@PVBC@IDA-Ni MNPs exhibit excellent performance in the separation of protein bovine hemoglobin (BHb), and the binding capacity is as high as 1988 mg g(-1). In addition, the Fe3O4@PVBC@IDA-Ni MNPs can be used in selective removal of abundant protein Hb in the bovine blood samples. This opens a novel route for its future application in removing abundant protein in proteomic analysis.
关键词[WOS]: HISTIDINE-TAGGED PROTEINS ;  CORE/SHELL NANOPARTICLES ;  BIOMEDICAL APPLICATIONS ;  MICROSPHERES ;  CHROMATOGRAPHY ;  EXTRACTION ;  SEPARATION ;  FE3O4/AU ;  SURFACE ;  SAMPLES
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000321626200001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119674
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Cao, Jiali,Zhang, Xihao,He, Xiwen,et al. Facile synthesis of a Ni(II)-immobilized core-shell magnetic nanocomposite as an efficient affinity adsorbent for the depletion of abundant proteins from bovine blood[J]. JOURNAL OF MATERIALS CHEMISTRY B,2013,1(30):3625-3632.
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