DICP OpenIR
Subject Area物理化学
Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions
Yang, F.1; Mao, J.1; He, X. W.1; Chen, L. X.1; Zhang, Y. K.1,2; Chen LX(陈朗星); Zhang YK(张玉奎)
KeywordBoronate Affinity Chromatography Hybrid Monolith Glycoprotein Physiological Ph Separation
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2013-08-01
DOI10.1007/s00216-013-7026-7
Volume405Issue:21Pages:6639-6648
Indexed BySCI
Cooperation Status
SubtypeArticle
Department18
Funding Project1810
Contribution Rank待补充
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Funding Organization2,5 ; 2,5 ; 2,5 ; 2,5
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordCAPILLARY LIQUID-CHROMATOGRAPHY ; MALDI-TOF MS ; CIS-DIOL ; MASS-SPECTROMETRY ; COLUMN ; GLYCOPEPTIDES ; ENRICHMENT ; GLYCOSYLATION ; SEPARATION ; ELECTROCHROMATOGRAPHY
AbstractIn this study, a boronate-silica hybrid affinity monolith was prepared for specific capture of glycoproteins at neutral pH condition. The monolith was synthesized via a facile one-pot procedure in a stainless steel column by concurrently mixing hydrolyzed alkoxysilanes tetramethoxysilane and vinyltrimethoxysilane, organic monomer 3-acrylamidophenylboronic acid and initiator 2,2'-azobisisobutyronitrile together. The polycondensation of alkoxysilanes and copolymerization of organic monomer and vinyl-silica monolith were carried out successively by reacting at different temperatures. After optimizing the preparation conditions, the resulting hybrid affinity monolith was systematically characterized and exhibited excellent affinity to both cis-diol-containing small molecules and glycoproteins at neutral and physiological pH, including adenosine, horseradish peroxidase, transferrin and ovalbumin. The binding capacity of ovalbumin on monolith was measured to be 2.5 mg g(-1) at pH 7.0. Furthermore, the hybrid affinity monolith was applied to the separation of transferrin from bovine serum sample at a physiological condition. Good repeatability was obtained and the relative standard deviations of retention time were 1.15 and 4.77 % (n = 5) for run-to-run and column-to-column, respectively.
Language英语
Funding Organization2,5 ; 2,5 ; 2,5 ; 2,5
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WOS IDWOS:000322705600004
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/119677
Collection中国科学院大连化学物理研究所
Corresponding AuthorChen LX(陈朗星); Zhang YK(张玉奎)
Affiliation1.Nankai Univ, State Key Lab Med Chem Biol, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Yang, F.,Mao, J.,He, X. W.,et al. Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(21):6639-6648.
APA Yang, F..,Mao, J..,He, X. W..,Chen, L. X..,Zhang, Y. K..,...&张玉奎.(2013).Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,405(21),6639-6648.
MLA Yang, F.,et al."Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 405.21(2013):6639-6648.
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