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学科主题物理化学
Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions
Yang, F.1; Mao, J.1; He, X. W.1; Chen, L. X.1; Zhang, Y. K.1,2; Chen LX(陈朗星); Zhang YK(张玉奎)
关键词Boronate Affinity Chromatography Hybrid Monolith Glycoprotein Physiological Ph Separation
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2013-08-01
DOI10.1007/s00216-013-7026-7
405期:21页:6639-6648
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1810
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
资助者2,5 ; 2,5 ; 2,5 ; 2,5
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CAPILLARY LIQUID-CHROMATOGRAPHY ; MALDI-TOF MS ; CIS-DIOL ; MASS-SPECTROMETRY ; COLUMN ; GLYCOPEPTIDES ; ENRICHMENT ; GLYCOSYLATION ; SEPARATION ; ELECTROCHROMATOGRAPHY
英文摘要In this study, a boronate-silica hybrid affinity monolith was prepared for specific capture of glycoproteins at neutral pH condition. The monolith was synthesized via a facile one-pot procedure in a stainless steel column by concurrently mixing hydrolyzed alkoxysilanes tetramethoxysilane and vinyltrimethoxysilane, organic monomer 3-acrylamidophenylboronic acid and initiator 2,2'-azobisisobutyronitrile together. The polycondensation of alkoxysilanes and copolymerization of organic monomer and vinyl-silica monolith were carried out successively by reacting at different temperatures. After optimizing the preparation conditions, the resulting hybrid affinity monolith was systematically characterized and exhibited excellent affinity to both cis-diol-containing small molecules and glycoproteins at neutral and physiological pH, including adenosine, horseradish peroxidase, transferrin and ovalbumin. The binding capacity of ovalbumin on monolith was measured to be 2.5 mg g(-1) at pH 7.0. Furthermore, the hybrid affinity monolith was applied to the separation of transferrin from bovine serum sample at a physiological condition. Good repeatability was obtained and the relative standard deviations of retention time were 1.15 and 4.77 % (n = 5) for run-to-run and column-to-column, respectively.
语种英语
资助者2,5 ; 2,5 ; 2,5 ; 2,5
原文出处查看原文
WOS记录号WOS:000322705600004
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119677
专题中国科学院大连化学物理研究所
通讯作者Chen LX(陈朗星); Zhang YK(张玉奎)
作者单位1.Nankai Univ, State Key Lab Med Chem Biol, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Yang, F.,Mao, J.,He, X. W.,et al. Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(21):6639-6648.
APA Yang, F..,Mao, J..,He, X. W..,Chen, L. X..,Zhang, Y. K..,...&张玉奎.(2013).Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,405(21),6639-6648.
MLA Yang, F.,et al."Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 405.21(2013):6639-6648.
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