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学科主题: 物理化学
题名: Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions
作者: Yang, F.1;  Mao, J.1;  He, X. W.1;  Chen, L. X.1;  Zhang, Y. K.1, 2
通讯作者: 陈朗星 ;  张玉奎
关键词: Boronate affinity chromatography ;  Hybrid monolith ;  Glycoprotein ;  Physiological pH ;  Separation
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2013-08-01
DOI: 10.1007/s00216-013-7026-7
卷: 405, 期:21, 页:6639-6648
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1810
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
资助者: 2,5
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this study, a boronate-silica hybrid affinity monolith was prepared for specific capture of glycoproteins at neutral pH condition. The monolith was synthesized via a facile one-pot procedure in a stainless steel column by concurrently mixing hydrolyzed alkoxysilanes tetramethoxysilane and vinyltrimethoxysilane, organic monomer 3-acrylamidophenylboronic acid and initiator 2,2'-azobisisobutyronitrile together. The polycondensation of alkoxysilanes and copolymerization of organic monomer and vinyl-silica monolith were carried out successively by reacting at different temperatures. After optimizing the preparation conditions, the resulting hybrid affinity monolith was systematically characterized and exhibited excellent affinity to both cis-diol-containing small molecules and glycoproteins at neutral and physiological pH, including adenosine, horseradish peroxidase, transferrin and ovalbumin. The binding capacity of ovalbumin on monolith was measured to be 2.5 mg g(-1) at pH 7.0. Furthermore, the hybrid affinity monolith was applied to the separation of transferrin from bovine serum sample at a physiological condition. Good repeatability was obtained and the relative standard deviations of retention time were 1.15 and 4.77 % (n = 5) for run-to-run and column-to-column, respectively.
关键词[WOS]: CAPILLARY LIQUID-CHROMATOGRAPHY ;  MALDI-TOF MS ;  CIS-DIOL ;  MASS-SPECTROMETRY ;  COLUMN ;  GLYCOPEPTIDES ;  ENRICHMENT ;  GLYCOSYLATION ;  SEPARATION ;  ELECTROCHROMATOGRAPHY
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000322705600004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119677
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Yang, F.,Mao, J.,He, X. W.,et al. Synthesis of boronate-silica hybrid affinity monolith via a one-pot process for specific capture of glycoproteins at neutral conditions[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2013,405(21):6639-6648.
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