中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome
作者: Bian, Yangyang1, 2;  Song, Chunxia1, 2;  Cheng, Kai1, 2;  Dong, Mingming1, 2;  Wang, Fangjun1;  Huang, Junfeng1, 2;  Sun, Deguang3;  Wang, Liming3;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 叶明亮 ;  邹汉法
关键词: Human liver phosphoproteome ;  RP-RPLC ;  Glu-C digestion ;  TripleTOF 5600 ;  LTQ Orbitrap Velos ;  Mass spectrometer
刊名: JOURNAL OF PROTEOMICS
发表日期: 2014-01-16
DOI: 10.1016/j.jprot.2013.11.014
卷: 96, 页:253-262
收录类别: SCI
合作性质: 
文章类型: Article
部门归属: 18
项目归属: 1809
产权排名: 待补充
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
资助者: 1,1
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Protein phosphorylation is one of the most common post-translational modifications. It plays key roles in regulating diverse biological processes of liver tissues. To better understand the role of protein phosphorylation in liver functions, it is essential to perform in-depth phosphoproteome analysis of human liver. Here, an enzyme assisted reversed-phase-reversed-phase liquid chromatography (RP-RPLC) approach with both RPLC separations operated with optimized acidic mobile phase was developed. High orthogonal separation was achieved by trypsin digestion of the Glu-C generated peptides in the fractions collected from the first RPLC separation. The phosphoproteome coverage was further improved by using two types of instruments, i.e. TripleTOF 5600 and LTQ Orbitrap Velos. A total of 22,446 phosphorylation sites, corresponding to 6526 nonredundant phosphoproteins were finally identified from normal human liver tissues. Of these sites, 15,229 sites were confidently localized with Ascore >= 13. This dataset was the largest phosphoproteome dataset of human liver. It can be a public resource for the liver research community and holds promise for further biology studies.
关键词[WOS]: ION AFFINITY-CHROMATOGRAPHY ;  PROTEIN IDENTIFICATION TECHNOLOGY ;  PHOSPHORYLATED PEPTIDES ;  MASS-SPECTROMETRY ;  MULTIDIMENSIONAL SEPARATION ;  ENRICHMENT ;  PROTEOMICS ;  PHOSPHOPEPTIDES ;  STRATEGY ;  NETWORKS
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000331916300020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/119681
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
2013zo81K963cw.PDF(445KB)----开放获取View 联系获取全文

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China

Recommended Citation:
Bian, Yangyang,Song, Chunxia,Cheng, Kai,et al. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome[J]. JOURNAL OF PROTEOMICS,2014,96:253-262.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Bian, Yangyang]'s Articles
 [Song, Chunxia]'s Articles
 [Cheng, Kai]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Bian, Yangyang]‘s Articles
 [Song, Chunxia]‘s Articles
 [Cheng, Kai]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2013zo81K963cw.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace