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学科主题物理化学
An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome
Bian, Yangyang1,2; Song, Chunxia1,2; Cheng, Kai1,2; Dong, Mingming1,2; Wang, Fangjun1; Huang, Junfeng1,2; Sun, Deguang3; Wang, Liming3; Ye, Mingliang1; Zou, Hanfa1; Ye ML(叶明亮); Zou HF(邹汉法)
关键词Human Liver Phosphoproteome Rp-rplc Glu-c Digestion Tripletof 5600 Ltq Orbitrap Velos Mass Spectrometer
刊名JOURNAL OF PROTEOMICS
2014-01-16
ISSN1874-3919
DOI10.1016/j.jprot.2013.11.014
96页:253-262
收录类别SCI
合作性质
文章类型Article
部门归属18
项目归属1809
产权排名待补充
WOS标题词Science & Technology ; Life Sciences & Biomedicine
资助者1,1 ; 1,1 ; 1,1 ; 1,1
类目[WOS]Biochemical Research Methods
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]ION AFFINITY-CHROMATOGRAPHY ; PROTEIN IDENTIFICATION TECHNOLOGY ; PHOSPHORYLATED PEPTIDES ; MASS-SPECTROMETRY ; MULTIDIMENSIONAL SEPARATION ; ENRICHMENT ; PROTEOMICS ; PHOSPHOPEPTIDES ; STRATEGY ; NETWORKS
英文摘要Protein phosphorylation is one of the most common post-translational modifications. It plays key roles in regulating diverse biological processes of liver tissues. To better understand the role of protein phosphorylation in liver functions, it is essential to perform in-depth phosphoproteome analysis of human liver. Here, an enzyme assisted reversed-phase-reversed-phase liquid chromatography (RP-RPLC) approach with both RPLC separations operated with optimized acidic mobile phase was developed. High orthogonal separation was achieved by trypsin digestion of the Glu-C generated peptides in the fractions collected from the first RPLC separation. The phosphoproteome coverage was further improved by using two types of instruments, i.e. TripleTOF 5600 and LTQ Orbitrap Velos. A total of 22,446 phosphorylation sites, corresponding to 6526 nonredundant phosphoproteins were finally identified from normal human liver tissues. Of these sites, 15,229 sites were confidently localized with Ascore >= 13. This dataset was the largest phosphoproteome dataset of human liver. It can be a public resource for the liver research community and holds promise for further biology studies.
语种英语
资助者1,1 ; 1,1 ; 1,1 ; 1,1
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WOS记录号WOS:000331916300020
引用统计
被引频次:85[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/119681
专题中国科学院大连化学物理研究所
通讯作者Ye ML(叶明亮); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China
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Bian, Yangyang,Song, Chunxia,Cheng, Kai,et al. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome[J]. JOURNAL OF PROTEOMICS,2014,96:253-262.
APA Bian, Yangyang.,Song, Chunxia.,Cheng, Kai.,Dong, Mingming.,Wang, Fangjun.,...&邹汉法.(2014).An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.JOURNAL OF PROTEOMICS,96,253-262.
MLA Bian, Yangyang,et al."An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome".JOURNAL OF PROTEOMICS 96(2014):253-262.
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